Spatiotemporal expression of periostin during skin development and incisional wound healing: Lessons for human fibrotic scar formation

Hong Ming Zhou, Jian Wang, Christopher Elliott, Weiyan Wen, Douglas W. Hamilton, Simon J. Conway

Research output: Contribution to journalArticle

67 Scopus citations

Abstract

Differentiation of fibroblasts to myofibroblasts and collagen fibrillogenesis are two processes essential for normal cutaneous development and repair, but their misregulation also underlies skin-associated fibrosis. Periostin is a matricellular protein normally expressed in adult skin, but its role in skin organogenesis, incisional wound healing and skin pathology has yet to be investigated in any depth. Using C57/BL6 mouse skin as model, we first investigated periostin protein and mRNA spatiotemporal expression and distribution during development and after incisional wounding. Secondarily we assessed whether periostin is expressed in human skin pathologies, including keloid and hypertrophic scars, psoriasis and atopic dermatitis. During development, periostin is expressed in the dermis, basement membrane and hair follicles from embryonic through neonatal stages and in the dermis and hair follicle only in adult. In situ hybridization demonstrated that dermal fibroblasts and basal keratinocytes express periostin mRNA. After incisional wounding, periostin becomes re-expressed in the basement membrane within the dermal-epidermal junction at the wound edge re-establishing the embryonic deposition pattern present in the adult. Analysis of periostin expression in human pathologies demonstrated that it is over-expressed in keloid and hypertrophic scars, atopic dermatitis, but is largely absent from sites of inflammation and inflammatory conditions such as psoriasis. Furthermore, in vitro we demonstrated that periostin is a transforming growth factor beta 1 inducible gene in human dermal fibroblasts. We conclude that periostin is an important ECM component during development, in wound healing and is strongly associated with pathological skin remodeling.

Original languageEnglish (US)
Pages (from-to)99-107
Number of pages9
JournalJournal of Cell Communication and Signaling
Volume4
Issue number2
DOIs
StatePublished - Jun 1 2010

Keywords

  • Development
  • Fibrosis
  • Pathology
  • Periostin
  • Skin
  • Wound healing

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Molecular Biology

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