Specificity and Expression of RalGPS as RalGEFs

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Ral proteins regulate a variety of biological processes and are major downstream targets of Ras because of association of the RalGDS family of exchange factors with Ras-GTP. However, a second, less-characterized family of Ral GEFs has also been isolated. Described here are methods used to determine the substrate specificity of this RalGPS family of GEFs, measure their membrane localization, interactions of with SH3 domains and phospholipids, as well as to measure their ability to activate Ral in vivo.

Original languageEnglish
Pages (from-to)108-114
Number of pages7
JournalMethods in Enzymology
Volume407
DOIs
StatePublished - 2005
Externally publishedYes

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ral GTP-Binding Proteins
Biological Phenomena
src Homology Domains
Substrate Specificity
Guanosine Triphosphate
Phospholipids
Membranes
Substrates

ASJC Scopus subject areas

  • Biochemistry

Cite this

Specificity and Expression of RalGPS as RalGEFs. / Quilliam, Lawrence.

In: Methods in Enzymology, Vol. 407, 2005, p. 108-114.

Research output: Contribution to journalArticle

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