Spermatocytic seminoma: An immunohistochemical study

Research output: Contribution to journalArticle

76 Citations (Scopus)

Abstract

Spermatocytic seminoma (SS) is an unusual germ cell tumor that behaves in an indolent fashion. Because orchiectomy alone is adequate treatment, it is important to distinguish SS from classic seminoma and other germ cell tumors. Light microscopic distinction usually is possible; however, occasional cases of SS exhibit atypical features, including the presence of a lymphoid infitrate or microcystic change, which simulate classic seminoma and yolk sac tumor, respectively. Immunohistochemistry might aid in this differential diagnosis, but the immunohistochemical profile of SS is not well reported in the literature. We examined seven SS cases (six men and one non-human primate) with a panel of 14 antibodies directed against placental-like alkaline phosphatase (PLAP), keratins (CAM 5.2, AE1 AE3), vimentin, human chorionic gonadotropin, alpha-fetoprotein, muscle-specific actin, carcinoembryonic antigen, S-100 protein, epithelial membrane antigen, desmin, leukocyte-common antigen, neuron-specific enolase, and human placental lactogen. A previously unreported finding was the presence of focal cytoplasmic staining for low molecular weight cytokeratin (CAM 5.2) in three cases. All other antibodies produced essentially negative results, including anti-PLAP. The PLAP and neuron-specific enolase negativity of SS are in contrast to the positivity of classic seminoma for these markers. A simplified panel of antibodies is recommended to assist in the differentiation of SS from other forms of germ cell neoplasia.

Original languageEnglish (US)
Pages (from-to)54-59
Number of pages6
JournalHuman pathology
Volume25
Issue number1
DOIs
StatePublished - Jan 1994

Fingerprint

Seminoma
S100 Proteins
Phosphopyruvate Hydratase
Germ Cell and Embryonal Neoplasms
Antibodies
CD45 Antigens
Endodermal Sinus Tumor
Placental Lactogen
Mucin-1
Desmin
Orchiectomy
Carcinoembryonic Antigen
alpha-Fetoproteins
Vimentin
Chorionic Gonadotropin
Keratins
Germ Cells
Primates
Actins
Differential Diagnosis

Keywords

  • alkaline phosphatase
  • germ cell tumors
  • immunoperoxidase
  • spermatocytic seminoma
  • testis

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Spermatocytic seminoma : An immunohistochemical study. / Cummings, Oscar W.; Ulbright, Thomas M.; Eble, John N.; Roth, Lawrence M.

In: Human pathology, Vol. 25, No. 1, 01.1994, p. 54-59.

Research output: Contribution to journalArticle

@article{063b4a542619400b8c10485da91b97e9,
title = "Spermatocytic seminoma: An immunohistochemical study",
abstract = "Spermatocytic seminoma (SS) is an unusual germ cell tumor that behaves in an indolent fashion. Because orchiectomy alone is adequate treatment, it is important to distinguish SS from classic seminoma and other germ cell tumors. Light microscopic distinction usually is possible; however, occasional cases of SS exhibit atypical features, including the presence of a lymphoid infitrate or microcystic change, which simulate classic seminoma and yolk sac tumor, respectively. Immunohistochemistry might aid in this differential diagnosis, but the immunohistochemical profile of SS is not well reported in the literature. We examined seven SS cases (six men and one non-human primate) with a panel of 14 antibodies directed against placental-like alkaline phosphatase (PLAP), keratins (CAM 5.2, AE1 AE3), vimentin, human chorionic gonadotropin, alpha-fetoprotein, muscle-specific actin, carcinoembryonic antigen, S-100 protein, epithelial membrane antigen, desmin, leukocyte-common antigen, neuron-specific enolase, and human placental lactogen. A previously unreported finding was the presence of focal cytoplasmic staining for low molecular weight cytokeratin (CAM 5.2) in three cases. All other antibodies produced essentially negative results, including anti-PLAP. The PLAP and neuron-specific enolase negativity of SS are in contrast to the positivity of classic seminoma for these markers. A simplified panel of antibodies is recommended to assist in the differentiation of SS from other forms of germ cell neoplasia.",
keywords = "alkaline phosphatase, germ cell tumors, immunoperoxidase, spermatocytic seminoma, testis",
author = "Cummings, {Oscar W.} and Ulbright, {Thomas M.} and Eble, {John N.} and Roth, {Lawrence M.}",
year = "1994",
month = "1",
doi = "10.1016/0046-8177(94)90171-6",
language = "English (US)",
volume = "25",
pages = "54--59",
journal = "Human Pathology",
issn = "0046-8177",
publisher = "W.B. Saunders Ltd",
number = "1",

}

TY - JOUR

T1 - Spermatocytic seminoma

T2 - An immunohistochemical study

AU - Cummings, Oscar W.

AU - Ulbright, Thomas M.

AU - Eble, John N.

AU - Roth, Lawrence M.

PY - 1994/1

Y1 - 1994/1

N2 - Spermatocytic seminoma (SS) is an unusual germ cell tumor that behaves in an indolent fashion. Because orchiectomy alone is adequate treatment, it is important to distinguish SS from classic seminoma and other germ cell tumors. Light microscopic distinction usually is possible; however, occasional cases of SS exhibit atypical features, including the presence of a lymphoid infitrate or microcystic change, which simulate classic seminoma and yolk sac tumor, respectively. Immunohistochemistry might aid in this differential diagnosis, but the immunohistochemical profile of SS is not well reported in the literature. We examined seven SS cases (six men and one non-human primate) with a panel of 14 antibodies directed against placental-like alkaline phosphatase (PLAP), keratins (CAM 5.2, AE1 AE3), vimentin, human chorionic gonadotropin, alpha-fetoprotein, muscle-specific actin, carcinoembryonic antigen, S-100 protein, epithelial membrane antigen, desmin, leukocyte-common antigen, neuron-specific enolase, and human placental lactogen. A previously unreported finding was the presence of focal cytoplasmic staining for low molecular weight cytokeratin (CAM 5.2) in three cases. All other antibodies produced essentially negative results, including anti-PLAP. The PLAP and neuron-specific enolase negativity of SS are in contrast to the positivity of classic seminoma for these markers. A simplified panel of antibodies is recommended to assist in the differentiation of SS from other forms of germ cell neoplasia.

AB - Spermatocytic seminoma (SS) is an unusual germ cell tumor that behaves in an indolent fashion. Because orchiectomy alone is adequate treatment, it is important to distinguish SS from classic seminoma and other germ cell tumors. Light microscopic distinction usually is possible; however, occasional cases of SS exhibit atypical features, including the presence of a lymphoid infitrate or microcystic change, which simulate classic seminoma and yolk sac tumor, respectively. Immunohistochemistry might aid in this differential diagnosis, but the immunohistochemical profile of SS is not well reported in the literature. We examined seven SS cases (six men and one non-human primate) with a panel of 14 antibodies directed against placental-like alkaline phosphatase (PLAP), keratins (CAM 5.2, AE1 AE3), vimentin, human chorionic gonadotropin, alpha-fetoprotein, muscle-specific actin, carcinoembryonic antigen, S-100 protein, epithelial membrane antigen, desmin, leukocyte-common antigen, neuron-specific enolase, and human placental lactogen. A previously unreported finding was the presence of focal cytoplasmic staining for low molecular weight cytokeratin (CAM 5.2) in three cases. All other antibodies produced essentially negative results, including anti-PLAP. The PLAP and neuron-specific enolase negativity of SS are in contrast to the positivity of classic seminoma for these markers. A simplified panel of antibodies is recommended to assist in the differentiation of SS from other forms of germ cell neoplasia.

KW - alkaline phosphatase

KW - germ cell tumors

KW - immunoperoxidase

KW - spermatocytic seminoma

KW - testis

UR - http://www.scopus.com/inward/record.url?scp=0028054739&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028054739&partnerID=8YFLogxK

U2 - 10.1016/0046-8177(94)90171-6

DO - 10.1016/0046-8177(94)90171-6

M3 - Article

C2 - 7508884

AN - SCOPUS:0028054739

VL - 25

SP - 54

EP - 59

JO - Human Pathology

JF - Human Pathology

SN - 0046-8177

IS - 1

ER -