Sphingosine-1-phosphate induces a PDGFR-dependent cell detachment via inhibiting β1 integrin in HEK293 cells

Alexander Zaslavsky, Song Li, Yan Xu

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Several different types of interactions between sphingosine-1-phosphate (S1P) receptors and platelet-derived growth factor receptor (PDGFR) have been revealed recently. In this work, we used HEK293 cells to further investigate the potential crosstalk. Interestingly, we observed that S1P specifically induced a PDGFR-dependent cell detachment in HEK293 cells, which could be inhibited by AG1296, a specific inhibitor for PDGFR. EGFR on the other hand, did not have any effect on cell detachment. The detachment was extracellular matrix (ECM) protein specific, suggesting the involvement of specific integrin molecules. When β1 integrin was engaged into an active state, S1P-induced cell detachment was blocked, suggesting that S1P induced an inside-out inhibitory effect on β1 integrin. Gi protein and ERK activation were required for the cell detachment induced by S1P, suggesting an endogenous receptor for S1P is likely to be involved.

Original languageEnglish (US)
Pages (from-to)3899-3906
Number of pages8
JournalFEBS Letters
Volume579
Issue number18
DOIs
StatePublished - Jul 18 2005
Externally publishedYes

Fingerprint

Platelet-Derived Growth Factor Receptors
HEK293 Cells
Integrins
Lysosphingolipid Receptors
Extracellular Matrix Proteins
Crosstalk
Chemical activation
Molecules
sphingosine 1-phosphate
Proteins

Keywords

  • β integrin
  • Cell detachment
  • Extracellular matrix
  • Inside-out inhibition
  • Platelet-derived growth factor receptor
  • Sphingosine-1-phosphate

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Sphingosine-1-phosphate induces a PDGFR-dependent cell detachment via inhibiting β1 integrin in HEK293 cells. / Zaslavsky, Alexander; Li, Song; Xu, Yan.

In: FEBS Letters, Vol. 579, No. 18, 18.07.2005, p. 3899-3906.

Research output: Contribution to journalArticle

@article{59a59a062cb143079fdc88b01077c85e,
title = "Sphingosine-1-phosphate induces a PDGFR-dependent cell detachment via inhibiting β1 integrin in HEK293 cells",
abstract = "Several different types of interactions between sphingosine-1-phosphate (S1P) receptors and platelet-derived growth factor receptor (PDGFR) have been revealed recently. In this work, we used HEK293 cells to further investigate the potential crosstalk. Interestingly, we observed that S1P specifically induced a PDGFR-dependent cell detachment in HEK293 cells, which could be inhibited by AG1296, a specific inhibitor for PDGFR. EGFR on the other hand, did not have any effect on cell detachment. The detachment was extracellular matrix (ECM) protein specific, suggesting the involvement of specific integrin molecules. When β1 integrin was engaged into an active state, S1P-induced cell detachment was blocked, suggesting that S1P induced an inside-out inhibitory effect on β1 integrin. Gi protein and ERK activation were required for the cell detachment induced by S1P, suggesting an endogenous receptor for S1P is likely to be involved.",
keywords = "β integrin, Cell detachment, Extracellular matrix, Inside-out inhibition, Platelet-derived growth factor receptor, Sphingosine-1-phosphate",
author = "Alexander Zaslavsky and Song Li and Yan Xu",
year = "2005",
month = "7",
day = "18",
doi = "10.1016/j.febslet.2005.06.006",
language = "English (US)",
volume = "579",
pages = "3899--3906",
journal = "FEBS Letters",
issn = "0014-5793",
publisher = "Elsevier",
number = "18",

}

TY - JOUR

T1 - Sphingosine-1-phosphate induces a PDGFR-dependent cell detachment via inhibiting β1 integrin in HEK293 cells

AU - Zaslavsky, Alexander

AU - Li, Song

AU - Xu, Yan

PY - 2005/7/18

Y1 - 2005/7/18

N2 - Several different types of interactions between sphingosine-1-phosphate (S1P) receptors and platelet-derived growth factor receptor (PDGFR) have been revealed recently. In this work, we used HEK293 cells to further investigate the potential crosstalk. Interestingly, we observed that S1P specifically induced a PDGFR-dependent cell detachment in HEK293 cells, which could be inhibited by AG1296, a specific inhibitor for PDGFR. EGFR on the other hand, did not have any effect on cell detachment. The detachment was extracellular matrix (ECM) protein specific, suggesting the involvement of specific integrin molecules. When β1 integrin was engaged into an active state, S1P-induced cell detachment was blocked, suggesting that S1P induced an inside-out inhibitory effect on β1 integrin. Gi protein and ERK activation were required for the cell detachment induced by S1P, suggesting an endogenous receptor for S1P is likely to be involved.

AB - Several different types of interactions between sphingosine-1-phosphate (S1P) receptors and platelet-derived growth factor receptor (PDGFR) have been revealed recently. In this work, we used HEK293 cells to further investigate the potential crosstalk. Interestingly, we observed that S1P specifically induced a PDGFR-dependent cell detachment in HEK293 cells, which could be inhibited by AG1296, a specific inhibitor for PDGFR. EGFR on the other hand, did not have any effect on cell detachment. The detachment was extracellular matrix (ECM) protein specific, suggesting the involvement of specific integrin molecules. When β1 integrin was engaged into an active state, S1P-induced cell detachment was blocked, suggesting that S1P induced an inside-out inhibitory effect on β1 integrin. Gi protein and ERK activation were required for the cell detachment induced by S1P, suggesting an endogenous receptor for S1P is likely to be involved.

KW - β integrin

KW - Cell detachment

KW - Extracellular matrix

KW - Inside-out inhibition

KW - Platelet-derived growth factor receptor

KW - Sphingosine-1-phosphate

UR - http://www.scopus.com/inward/record.url?scp=21844456516&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=21844456516&partnerID=8YFLogxK

U2 - 10.1016/j.febslet.2005.06.006

DO - 10.1016/j.febslet.2005.06.006

M3 - Article

C2 - 15987639

AN - SCOPUS:21844456516

VL - 579

SP - 3899

EP - 3906

JO - FEBS Letters

JF - FEBS Letters

SN - 0014-5793

IS - 18

ER -