Sphingosylphosphorylcholine is a ligand for ovarian cancer G-protein-coupled receptor 1

Yan Xu, Kui Zhu, Guiying Hong, Weihua Wu, Linnea M. Baudhuin, Yi Jin Xiao, Derek S. Damron

Research output: Contribution to journalArticle

166 Citations (Scopus)

Abstract

Sphingosylphosphorylcholine (SPC) is a bioactive lipid that acts as an intracellular and extracellular signalling molecule in numerous biological processes. Many of the cellular actions of SPC are believed to be mediated by the activation of unidentified G-protein-coupled receptors. Here we sinew that SPC is a high-affinity ligand for an orphan receptor, ovarian cancer G-protein-coupled receptor 1 (OGR1). In OGR1-transfected cells, SPC binds to OGR1 with high affinity (K(d) = 33.3 nM) and high specificity and transiently increases intracellular calcium. The specific binding of SPC to OGR1 also activates p42/44 mitogen-activated protein kinases (MAP kinases) and inhibits cell proliferation. In addition, SPC causes internalization of OGR1 in a structurally specific manner.

Original languageEnglish (US)
Pages (from-to)261-267
Number of pages7
JournalNature Cell Biology
Volume2
Issue number5
DOIs
StatePublished - 2000
Externally publishedYes

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G-Protein-Coupled Receptors
Ovarian Neoplasms
Ligands
Biological Phenomena
Mitogen-Activated Protein Kinase 1
sphingosine phosphorylcholine
Cell Proliferation
Calcium
Lipids

ASJC Scopus subject areas

  • Cell Biology

Cite this

Xu, Y., Zhu, K., Hong, G., Wu, W., Baudhuin, L. M., Xiao, Y. J., & Damron, D. S. (2000). Sphingosylphosphorylcholine is a ligand for ovarian cancer G-protein-coupled receptor 1. Nature Cell Biology, 2(5), 261-267. https://doi.org/10.1038/35010529

Sphingosylphosphorylcholine is a ligand for ovarian cancer G-protein-coupled receptor 1. / Xu, Yan; Zhu, Kui; Hong, Guiying; Wu, Weihua; Baudhuin, Linnea M.; Xiao, Yi Jin; Damron, Derek S.

In: Nature Cell Biology, Vol. 2, No. 5, 2000, p. 261-267.

Research output: Contribution to journalArticle

Xu, Y, Zhu, K, Hong, G, Wu, W, Baudhuin, LM, Xiao, YJ & Damron, DS 2000, 'Sphingosylphosphorylcholine is a ligand for ovarian cancer G-protein-coupled receptor 1', Nature Cell Biology, vol. 2, no. 5, pp. 261-267. https://doi.org/10.1038/35010529
Xu, Yan ; Zhu, Kui ; Hong, Guiying ; Wu, Weihua ; Baudhuin, Linnea M. ; Xiao, Yi Jin ; Damron, Derek S. / Sphingosylphosphorylcholine is a ligand for ovarian cancer G-protein-coupled receptor 1. In: Nature Cell Biology. 2000 ; Vol. 2, No. 5. pp. 261-267.
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