Spinal cord lateral hemisection and implantation of guidance channels

Xiaofei Wang, Xiao Ming Xu

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Spinal cord laceration injuries such as complete transection, lateral and dorsal hemisection, over hemisection, and dorsal funiculotomy in rodents have been widely used in the studies of neuronal responses to axotomy as well as axonal regeneration through and beyond the site of the injury. The latter approach has utilized transplantation of fetal cord tissues, pieces of peripheral nerves, and biosynthetic guidance channels seeded with different cell types, trophic factors, and matrix molecules. In this chapter, we introduce hands-on procedures to perform a spinal cord lateral hemisection, create a lesion cavity on the same side, and transplant a piece of guidance channel that bridges the two ends of the cord stumps. By suturing the dura, cerebrospinal fluid circulation is restored and connective tissue invasion into the lesion site is prevented. With modification, these procedures should also apply to creating abovementioned laceration injuries when microscissors are used.

Original languageEnglish (US)
Title of host publicationAnimal Models of Acute Neurological Injuries
PublisherHumana Press
Pages479-486
Number of pages8
ISBN (Print)9781603271844
DOIs
StatePublished - Dec 1 2009

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Keywords

  • Guidance channel
  • Hemisection
  • Laceration
  • Spinal cord injury
  • Transection
  • Transplantation

ASJC Scopus subject areas

  • Neuroscience(all)
  • Agricultural and Biological Sciences(all)

Cite this

Wang, X., & Xu, X. M. (2009). Spinal cord lateral hemisection and implantation of guidance channels. In Animal Models of Acute Neurological Injuries (pp. 479-486). Humana Press. https://doi.org/10.1007/978-1-60327-185-1_41