Splenic macrophages: mediators of immunosuppressive activity of staphylococcal peptidoglycan

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

The mechanism of the immunosuppressive effect of staphylococcal peptidoglycan (PG) on the primary antibody (Ab) response to sheep erythrocytes in mice was studied. The immunosuppression could be transferred into normal recipients by syngeneic spleen cells from PG-treated mice, while transfer of spleen cell sonicates was not effective. In vitro immunization of dispersed spleen cell cultures revealed that anti-erythrocyte response of splenocytes from PG-treated mice was substantially lower than the Ab response of splenocytes from untreated animals. Ten to 20% of spleen cells from PG injected mice suppressed the Ab response of normal splenocytes when added at the initiation of the cultures. Living cells were required for the effective suppression, and PG-induced suppressor cells interfered with the early stage of the immune response. Cell fractionation revealed that nonadherent spleen cells from PG-treated mice were not immunosuppressive for normal cells. Adherent cells from PG-injected animals were immunosuppressive at very low concentrations (2%). At higher concentrations (20%) adherent cells from untreated mice were also immunosuppressive. The adherent cell population comprised 90% macrophages. These cells could be made immunosuppressive by in vitro treatment with PG. This suggested direct induction of immunosuppressive cells by PG.

Original languageEnglish (US)
Pages (from-to)239-247
Number of pages9
JournalRES Journal of the Reticuloendothelial Society
Volume26
Issue number3
StatePublished - 1979
Externally publishedYes

Fingerprint

Peptidoglycan
Immunosuppressive Agents
Macrophages
Spleen
Antibody Formation
Erythrocytes
Cell Fractionation
Immunosuppression
Immunization
Sheep
Cell Culture Techniques

ASJC Scopus subject areas

  • Hematology

Cite this

@article{12ee66444308494ba9546d4d17c53d6c,
title = "Splenic macrophages: mediators of immunosuppressive activity of staphylococcal peptidoglycan",
abstract = "The mechanism of the immunosuppressive effect of staphylococcal peptidoglycan (PG) on the primary antibody (Ab) response to sheep erythrocytes in mice was studied. The immunosuppression could be transferred into normal recipients by syngeneic spleen cells from PG-treated mice, while transfer of spleen cell sonicates was not effective. In vitro immunization of dispersed spleen cell cultures revealed that anti-erythrocyte response of splenocytes from PG-treated mice was substantially lower than the Ab response of splenocytes from untreated animals. Ten to 20{\%} of spleen cells from PG injected mice suppressed the Ab response of normal splenocytes when added at the initiation of the cultures. Living cells were required for the effective suppression, and PG-induced suppressor cells interfered with the early stage of the immune response. Cell fractionation revealed that nonadherent spleen cells from PG-treated mice were not immunosuppressive for normal cells. Adherent cells from PG-injected animals were immunosuppressive at very low concentrations (2{\%}). At higher concentrations (20{\%}) adherent cells from untreated mice were also immunosuppressive. The adherent cell population comprised 90{\%} macrophages. These cells could be made immunosuppressive by in vitro treatment with PG. This suggested direct induction of immunosuppressive cells by PG.",
author = "Roman Dziarski",
year = "1979",
language = "English (US)",
volume = "26",
pages = "239--247",
journal = "Journal of Leukocyte Biology",
issn = "0741-5400",
publisher = "FASEB",
number = "3",

}

TY - JOUR

T1 - Splenic macrophages

T2 - mediators of immunosuppressive activity of staphylococcal peptidoglycan

AU - Dziarski, Roman

PY - 1979

Y1 - 1979

N2 - The mechanism of the immunosuppressive effect of staphylococcal peptidoglycan (PG) on the primary antibody (Ab) response to sheep erythrocytes in mice was studied. The immunosuppression could be transferred into normal recipients by syngeneic spleen cells from PG-treated mice, while transfer of spleen cell sonicates was not effective. In vitro immunization of dispersed spleen cell cultures revealed that anti-erythrocyte response of splenocytes from PG-treated mice was substantially lower than the Ab response of splenocytes from untreated animals. Ten to 20% of spleen cells from PG injected mice suppressed the Ab response of normal splenocytes when added at the initiation of the cultures. Living cells were required for the effective suppression, and PG-induced suppressor cells interfered with the early stage of the immune response. Cell fractionation revealed that nonadherent spleen cells from PG-treated mice were not immunosuppressive for normal cells. Adherent cells from PG-injected animals were immunosuppressive at very low concentrations (2%). At higher concentrations (20%) adherent cells from untreated mice were also immunosuppressive. The adherent cell population comprised 90% macrophages. These cells could be made immunosuppressive by in vitro treatment with PG. This suggested direct induction of immunosuppressive cells by PG.

AB - The mechanism of the immunosuppressive effect of staphylococcal peptidoglycan (PG) on the primary antibody (Ab) response to sheep erythrocytes in mice was studied. The immunosuppression could be transferred into normal recipients by syngeneic spleen cells from PG-treated mice, while transfer of spleen cell sonicates was not effective. In vitro immunization of dispersed spleen cell cultures revealed that anti-erythrocyte response of splenocytes from PG-treated mice was substantially lower than the Ab response of splenocytes from untreated animals. Ten to 20% of spleen cells from PG injected mice suppressed the Ab response of normal splenocytes when added at the initiation of the cultures. Living cells were required for the effective suppression, and PG-induced suppressor cells interfered with the early stage of the immune response. Cell fractionation revealed that nonadherent spleen cells from PG-treated mice were not immunosuppressive for normal cells. Adherent cells from PG-injected animals were immunosuppressive at very low concentrations (2%). At higher concentrations (20%) adherent cells from untreated mice were also immunosuppressive. The adherent cell population comprised 90% macrophages. These cells could be made immunosuppressive by in vitro treatment with PG. This suggested direct induction of immunosuppressive cells by PG.

UR - http://www.scopus.com/inward/record.url?scp=0018731238&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0018731238&partnerID=8YFLogxK

M3 - Article

C2 - 501708

AN - SCOPUS:0018731238

VL - 26

SP - 239

EP - 247

JO - Journal of Leukocyte Biology

JF - Journal of Leukocyte Biology

SN - 0741-5400

IS - 3

ER -