Spontaneous proliferation and type 2 cytokine secretion by CD4+T cells in patients with metastatic melanoma vaccinated with antigen-pulsed dendritic cells

A. Karolina Palucka, John Connolly, Hideki Ueno, John Kohl, Sophie Paczesny, Madhav Dhodapkar, Joseph Fay, Jacques Banchereau

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

We observed the induction of spontaneous, i.e., without adding exogenous antigen, in vitro proliferation of PBMCs from patients with stage IV melanoma who underwent repeated vaccinations with antigen-loaded dendritic cells (DCs) derived from CD34+ hematopoietic progenitors (CD34-DCs). Proliferating cells are CD4+T cells. Their proliferation is dependent on (1) CD11c+ myeloid DCs, since their depletion from PBMCs abolishes it; and (2) IL-2, as it can be blocked by neutralizing anti-IL-2 antibodies. Spontaneous proliferation is associated to the secretion of type 2 cytokines. To analyze the frequency of spontaneous proliferation induction in the cohort of 18 vaccinated patients, an index of spontaneous proliferation was defined as a ratio of PBMCs proliferation from post- vs pre-DC vaccination blood samples. Ten out of sixteen analyzed patients showed an index >2. The index of spontaneous proliferation correlates with antigen-specific PBMC proliferation to the vaccine antigen KLH. Furthermore, both spontaneous- and antigen-specific proliferation in PBMC cultures are dependent on blood myeloid DCs.

Original languageEnglish (US)
Pages (from-to)288-295
Number of pages8
JournalJournal of Clinical Immunology
Volume25
Issue number3
DOIs
StatePublished - May 2005
Externally publishedYes

Fingerprint

Dendritic Cells
Melanoma
Cytokines
T-Lymphocytes
Antigens
Myeloid Cells
Interleukin-2
Vaccination
Blood Cells
Vaccines
Antibodies

Keywords

  • Cytokines
  • T cell immunity
  • Tumor immunity
  • Vaccination

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

Spontaneous proliferation and type 2 cytokine secretion by CD4+T cells in patients with metastatic melanoma vaccinated with antigen-pulsed dendritic cells. / Palucka, A. Karolina; Connolly, John; Ueno, Hideki; Kohl, John; Paczesny, Sophie; Dhodapkar, Madhav; Fay, Joseph; Banchereau, Jacques.

In: Journal of Clinical Immunology, Vol. 25, No. 3, 05.2005, p. 288-295.

Research output: Contribution to journalArticle

Palucka, A. Karolina ; Connolly, John ; Ueno, Hideki ; Kohl, John ; Paczesny, Sophie ; Dhodapkar, Madhav ; Fay, Joseph ; Banchereau, Jacques. / Spontaneous proliferation and type 2 cytokine secretion by CD4+T cells in patients with metastatic melanoma vaccinated with antigen-pulsed dendritic cells. In: Journal of Clinical Immunology. 2005 ; Vol. 25, No. 3. pp. 288-295.
@article{864dd1ee41384198b70f9ef221384c4e,
title = "Spontaneous proliferation and type 2 cytokine secretion by CD4+T cells in patients with metastatic melanoma vaccinated with antigen-pulsed dendritic cells",
abstract = "We observed the induction of spontaneous, i.e., without adding exogenous antigen, in vitro proliferation of PBMCs from patients with stage IV melanoma who underwent repeated vaccinations with antigen-loaded dendritic cells (DCs) derived from CD34+ hematopoietic progenitors (CD34-DCs). Proliferating cells are CD4+T cells. Their proliferation is dependent on (1) CD11c+ myeloid DCs, since their depletion from PBMCs abolishes it; and (2) IL-2, as it can be blocked by neutralizing anti-IL-2 antibodies. Spontaneous proliferation is associated to the secretion of type 2 cytokines. To analyze the frequency of spontaneous proliferation induction in the cohort of 18 vaccinated patients, an index of spontaneous proliferation was defined as a ratio of PBMCs proliferation from post- vs pre-DC vaccination blood samples. Ten out of sixteen analyzed patients showed an index >2. The index of spontaneous proliferation correlates with antigen-specific PBMC proliferation to the vaccine antigen KLH. Furthermore, both spontaneous- and antigen-specific proliferation in PBMC cultures are dependent on blood myeloid DCs.",
keywords = "Cytokines, T cell immunity, Tumor immunity, Vaccination",
author = "Palucka, {A. Karolina} and John Connolly and Hideki Ueno and John Kohl and Sophie Paczesny and Madhav Dhodapkar and Joseph Fay and Jacques Banchereau",
year = "2005",
month = "5",
doi = "10.1007/s10875-005-4089-z",
language = "English (US)",
volume = "25",
pages = "288--295",
journal = "Journal of Clinical Immunology",
issn = "0271-9142",
publisher = "Springer New York",
number = "3",

}

TY - JOUR

T1 - Spontaneous proliferation and type 2 cytokine secretion by CD4+T cells in patients with metastatic melanoma vaccinated with antigen-pulsed dendritic cells

AU - Palucka, A. Karolina

AU - Connolly, John

AU - Ueno, Hideki

AU - Kohl, John

AU - Paczesny, Sophie

AU - Dhodapkar, Madhav

AU - Fay, Joseph

AU - Banchereau, Jacques

PY - 2005/5

Y1 - 2005/5

N2 - We observed the induction of spontaneous, i.e., without adding exogenous antigen, in vitro proliferation of PBMCs from patients with stage IV melanoma who underwent repeated vaccinations with antigen-loaded dendritic cells (DCs) derived from CD34+ hematopoietic progenitors (CD34-DCs). Proliferating cells are CD4+T cells. Their proliferation is dependent on (1) CD11c+ myeloid DCs, since their depletion from PBMCs abolishes it; and (2) IL-2, as it can be blocked by neutralizing anti-IL-2 antibodies. Spontaneous proliferation is associated to the secretion of type 2 cytokines. To analyze the frequency of spontaneous proliferation induction in the cohort of 18 vaccinated patients, an index of spontaneous proliferation was defined as a ratio of PBMCs proliferation from post- vs pre-DC vaccination blood samples. Ten out of sixteen analyzed patients showed an index >2. The index of spontaneous proliferation correlates with antigen-specific PBMC proliferation to the vaccine antigen KLH. Furthermore, both spontaneous- and antigen-specific proliferation in PBMC cultures are dependent on blood myeloid DCs.

AB - We observed the induction of spontaneous, i.e., without adding exogenous antigen, in vitro proliferation of PBMCs from patients with stage IV melanoma who underwent repeated vaccinations with antigen-loaded dendritic cells (DCs) derived from CD34+ hematopoietic progenitors (CD34-DCs). Proliferating cells are CD4+T cells. Their proliferation is dependent on (1) CD11c+ myeloid DCs, since their depletion from PBMCs abolishes it; and (2) IL-2, as it can be blocked by neutralizing anti-IL-2 antibodies. Spontaneous proliferation is associated to the secretion of type 2 cytokines. To analyze the frequency of spontaneous proliferation induction in the cohort of 18 vaccinated patients, an index of spontaneous proliferation was defined as a ratio of PBMCs proliferation from post- vs pre-DC vaccination blood samples. Ten out of sixteen analyzed patients showed an index >2. The index of spontaneous proliferation correlates with antigen-specific PBMC proliferation to the vaccine antigen KLH. Furthermore, both spontaneous- and antigen-specific proliferation in PBMC cultures are dependent on blood myeloid DCs.

KW - Cytokines

KW - T cell immunity

KW - Tumor immunity

KW - Vaccination

UR - http://www.scopus.com/inward/record.url?scp=21244506203&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=21244506203&partnerID=8YFLogxK

U2 - 10.1007/s10875-005-4089-z

DO - 10.1007/s10875-005-4089-z

M3 - Article

VL - 25

SP - 288

EP - 295

JO - Journal of Clinical Immunology

JF - Journal of Clinical Immunology

SN - 0271-9142

IS - 3

ER -