Abstract
In this study, we isolated and characterized spontaneously differentiated human embryonic stem cells (SD-hESCs) found in hESC colonies in comparison to the morphologically premature ESCs in the colonies to investigate the potential role of SD-hESCs in embryogenesis. SD-hESCs were distinguished from undifferentiated hESCs by their higher expression of GATA6, a marker for primitive endoderm and transthyretin, a marker visceral endoderm in embryoid bodies (EBs). SD-hESCs expressed OCT4 and NANOG, markers for pluripotent stem cells, at significantly lower levels than undifferentiated hESCs. EBs derived from isolated SD-hESCs were morphologically distinct from cells directly derived from the undifferentiated hESCs; they contained higher number of cysts compared to EBs from undifferentiated hESC-derived EBs (42% vs. 20%). Furthermore, the extracellular signal molecule, BMP2/4, induced a higher GATA4/6 expression and cystic EB formation than control and noggin-treated EBs. Since cystic formation in EBs play a role in primitive endoderm formation during embryogenesis, the SD-hESC may be a relevant cell type equipped to differentiate into primitive endoderm. Our results suggest that SD-ESCs generated during routine hESC culture are not just an artifact of in vitro culture and these cells could serve as a useful model to study the process of embryogenesis.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 2706-2713 |
| Number of pages | 8 |
| Journal | Stem Cells and Development |
| Volume | 22 |
| Issue number | 20 |
| DOIs | |
| State | Published - Oct 15 2013 |
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ASJC Scopus subject areas
- Cell Biology
- Developmental Biology
- Hematology
Cite this
Spontaneously differentiated GATA6-positive human embryonic stem cells represent an important cellular step in human embryonic development; They are not just an artifact of in vitro culture. / Lee, Jun Ho; Hong, Ki Sung; Mantel, Charlie; Broxmeyer, Hal E.; Lee, Man Ryul; Kim, Kye Seong.
In: Stem Cells and Development, Vol. 22, No. 20, 15.10.2013, p. 2706-2713.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Spontaneously differentiated GATA6-positive human embryonic stem cells represent an important cellular step in human embryonic development; They are not just an artifact of in vitro culture
AU - Lee, Jun Ho
AU - Hong, Ki Sung
AU - Mantel, Charlie
AU - Broxmeyer, Hal E.
AU - Lee, Man Ryul
AU - Kim, Kye Seong
PY - 2013/10/15
Y1 - 2013/10/15
N2 - In this study, we isolated and characterized spontaneously differentiated human embryonic stem cells (SD-hESCs) found in hESC colonies in comparison to the morphologically premature ESCs in the colonies to investigate the potential role of SD-hESCs in embryogenesis. SD-hESCs were distinguished from undifferentiated hESCs by their higher expression of GATA6, a marker for primitive endoderm and transthyretin, a marker visceral endoderm in embryoid bodies (EBs). SD-hESCs expressed OCT4 and NANOG, markers for pluripotent stem cells, at significantly lower levels than undifferentiated hESCs. EBs derived from isolated SD-hESCs were morphologically distinct from cells directly derived from the undifferentiated hESCs; they contained higher number of cysts compared to EBs from undifferentiated hESC-derived EBs (42% vs. 20%). Furthermore, the extracellular signal molecule, BMP2/4, induced a higher GATA4/6 expression and cystic EB formation than control and noggin-treated EBs. Since cystic formation in EBs play a role in primitive endoderm formation during embryogenesis, the SD-hESC may be a relevant cell type equipped to differentiate into primitive endoderm. Our results suggest that SD-ESCs generated during routine hESC culture are not just an artifact of in vitro culture and these cells could serve as a useful model to study the process of embryogenesis.
AB - In this study, we isolated and characterized spontaneously differentiated human embryonic stem cells (SD-hESCs) found in hESC colonies in comparison to the morphologically premature ESCs in the colonies to investigate the potential role of SD-hESCs in embryogenesis. SD-hESCs were distinguished from undifferentiated hESCs by their higher expression of GATA6, a marker for primitive endoderm and transthyretin, a marker visceral endoderm in embryoid bodies (EBs). SD-hESCs expressed OCT4 and NANOG, markers for pluripotent stem cells, at significantly lower levels than undifferentiated hESCs. EBs derived from isolated SD-hESCs were morphologically distinct from cells directly derived from the undifferentiated hESCs; they contained higher number of cysts compared to EBs from undifferentiated hESC-derived EBs (42% vs. 20%). Furthermore, the extracellular signal molecule, BMP2/4, induced a higher GATA4/6 expression and cystic EB formation than control and noggin-treated EBs. Since cystic formation in EBs play a role in primitive endoderm formation during embryogenesis, the SD-hESC may be a relevant cell type equipped to differentiate into primitive endoderm. Our results suggest that SD-ESCs generated during routine hESC culture are not just an artifact of in vitro culture and these cells could serve as a useful model to study the process of embryogenesis.
UR - http://www.scopus.com/inward/record.url?scp=84885150714&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84885150714&partnerID=8YFLogxK
U2 - 10.1089/scd.2013.0083
DO - 10.1089/scd.2013.0083
M3 - Article
C2 - 23746070
AN - SCOPUS:84885150714
VL - 22
SP - 2706
EP - 2713
JO - Stem Cells and Development
JF - Stem Cells and Development
SN - 1547-3287
IS - 20
ER -