Src Phosphorylates Cas on Tyrosine 253 to Promote Migration of Transformed Cells

Gary S. Goldberg, David B. Alexander, Patricia Pellicena, Zhong Yin Zhang, Hiroyuki Tsuda, W. Todd Miller

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Abstract

Cas is a member of the focal adhesion complex. Phosphorylation of Cas by Src is an important event leading to cell transformation. Using mass spectrometry, we have mapped 11 sites in Cas that are phosphorylated by Src. These sites are all located between residues 132 and 414 of Cas, in a region that is required for binding to a number of other proteins including Crk. We tested synthetic peptides modeled on Cas phosphorylation sites, and found that the sequence containing tyrosine 253 was phosphorylated by Src most efficiently. Using cells derived from Cas-deficient mice, we confirmed that Cas greatly enhanced the ability of Src to transform cells. Phosphorylation of Cas on tyrosine 253 was not required for Src to increase growth rate, suppress contact inhibition, or suppress anchorage dependence. Yet, in contrast to these growth characteristics, phosphorylation of Cas on tyrosine 253 was required for Src to promote cell migration. Thus, a single phosphorylation site on this focal adhesion adaptor protein can effectively separate cell migration from other transformed growth characteristics.

Original languageEnglish (US)
Pages (from-to)46533-46540
Number of pages8
JournalJournal of Biological Chemistry
Volume278
Issue number47
DOIs
StatePublished - Nov 21 2003

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ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Goldberg, G. S., Alexander, D. B., Pellicena, P., Zhang, Z. Y., Tsuda, H., & Miller, W. T. (2003). Src Phosphorylates Cas on Tyrosine 253 to Promote Migration of Transformed Cells. Journal of Biological Chemistry, 278(47), 46533-46540. https://doi.org/10.1074/jbc.M307526200