Stability of interferon-gamma and interleukin-10 responses to Plasmodium falciparum liver stage antigen 1 and thrombospondin-related adhesive protein immunodominant epitopes in a highland population from Western Kenya

Ann M. Moormann, Peter Odada Sumba, Daniel J. Tisch, Paula Embury, Charles H. King, James W. Kazura, Chandy John

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Long-term planning to prevent malaria epidemics requires in-depth understanding of immunity to Plasmodium falciparum in areas of unstable transmission. Cytokine responses to immunodominant epitope peptides from liver stage antigen 1 (LSA-1) and thrombospondin-related adhesive protein (TRAP) were evaluated over a nine-month interval in adults and children in Kenya from a malaria epidemic-prone highland area after several years of low transmission. The proportion and magnitude of interferon-gamma ELISPOT responses and the proportion of interleukin-10 responders to LSA-1 and TRAP peptides tended to be higher in adults than children. Frequencies of interferon-gamma responders to these peptides were similar at the two time points, but responses were not consistently generated by the same persons. These results suggest that T cell memory to prc-erythrocytic stage malaria antigens is maintained but may be unavailable for consistent detection in peripheral blood, and that these antigens induce both pro-inflammatory and anti-inflammatory cytokine responses in this population.

Original languageEnglish (US)
Pages (from-to)489-495
Number of pages7
JournalAmerican Journal of Tropical Medicine and Hygiene
Volume81
Issue number3
StatePublished - Sep 2009
Externally publishedYes

Fingerprint

Thrombospondin 1
Immunodominant Epitopes
Kenya
Plasmodium falciparum
Adhesives
Interleukin-10
Interferon-gamma
Malaria
Antigens
Liver
Peptides
Population
Proteins
Cytokines
Enzyme-Linked Immunospot Assay
Immunity
Anti-Inflammatory Agents
T-Lymphocytes

ASJC Scopus subject areas

  • Parasitology
  • Infectious Diseases
  • Virology

Cite this

Stability of interferon-gamma and interleukin-10 responses to Plasmodium falciparum liver stage antigen 1 and thrombospondin-related adhesive protein immunodominant epitopes in a highland population from Western Kenya. / Moormann, Ann M.; Sumba, Peter Odada; Tisch, Daniel J.; Embury, Paula; King, Charles H.; Kazura, James W.; John, Chandy.

In: American Journal of Tropical Medicine and Hygiene, Vol. 81, No. 3, 09.2009, p. 489-495.

Research output: Contribution to journalArticle

@article{33a0e4ab460a40748388143187913d61,
title = "Stability of interferon-gamma and interleukin-10 responses to Plasmodium falciparum liver stage antigen 1 and thrombospondin-related adhesive protein immunodominant epitopes in a highland population from Western Kenya",
abstract = "Long-term planning to prevent malaria epidemics requires in-depth understanding of immunity to Plasmodium falciparum in areas of unstable transmission. Cytokine responses to immunodominant epitope peptides from liver stage antigen 1 (LSA-1) and thrombospondin-related adhesive protein (TRAP) were evaluated over a nine-month interval in adults and children in Kenya from a malaria epidemic-prone highland area after several years of low transmission. The proportion and magnitude of interferon-gamma ELISPOT responses and the proportion of interleukin-10 responders to LSA-1 and TRAP peptides tended to be higher in adults than children. Frequencies of interferon-gamma responders to these peptides were similar at the two time points, but responses were not consistently generated by the same persons. These results suggest that T cell memory to prc-erythrocytic stage malaria antigens is maintained but may be unavailable for consistent detection in peripheral blood, and that these antigens induce both pro-inflammatory and anti-inflammatory cytokine responses in this population.",
author = "Moormann, {Ann M.} and Sumba, {Peter Odada} and Tisch, {Daniel J.} and Paula Embury and King, {Charles H.} and Kazura, {James W.} and Chandy John",
year = "2009",
month = "9",
language = "English (US)",
volume = "81",
pages = "489--495",
journal = "American Journal of Tropical Medicine and Hygiene",
issn = "0002-9637",
publisher = "American Society of Tropical Medicine and Hygiene",
number = "3",

}

TY - JOUR

T1 - Stability of interferon-gamma and interleukin-10 responses to Plasmodium falciparum liver stage antigen 1 and thrombospondin-related adhesive protein immunodominant epitopes in a highland population from Western Kenya

AU - Moormann, Ann M.

AU - Sumba, Peter Odada

AU - Tisch, Daniel J.

AU - Embury, Paula

AU - King, Charles H.

AU - Kazura, James W.

AU - John, Chandy

PY - 2009/9

Y1 - 2009/9

N2 - Long-term planning to prevent malaria epidemics requires in-depth understanding of immunity to Plasmodium falciparum in areas of unstable transmission. Cytokine responses to immunodominant epitope peptides from liver stage antigen 1 (LSA-1) and thrombospondin-related adhesive protein (TRAP) were evaluated over a nine-month interval in adults and children in Kenya from a malaria epidemic-prone highland area after several years of low transmission. The proportion and magnitude of interferon-gamma ELISPOT responses and the proportion of interleukin-10 responders to LSA-1 and TRAP peptides tended to be higher in adults than children. Frequencies of interferon-gamma responders to these peptides were similar at the two time points, but responses were not consistently generated by the same persons. These results suggest that T cell memory to prc-erythrocytic stage malaria antigens is maintained but may be unavailable for consistent detection in peripheral blood, and that these antigens induce both pro-inflammatory and anti-inflammatory cytokine responses in this population.

AB - Long-term planning to prevent malaria epidemics requires in-depth understanding of immunity to Plasmodium falciparum in areas of unstable transmission. Cytokine responses to immunodominant epitope peptides from liver stage antigen 1 (LSA-1) and thrombospondin-related adhesive protein (TRAP) were evaluated over a nine-month interval in adults and children in Kenya from a malaria epidemic-prone highland area after several years of low transmission. The proportion and magnitude of interferon-gamma ELISPOT responses and the proportion of interleukin-10 responders to LSA-1 and TRAP peptides tended to be higher in adults than children. Frequencies of interferon-gamma responders to these peptides were similar at the two time points, but responses were not consistently generated by the same persons. These results suggest that T cell memory to prc-erythrocytic stage malaria antigens is maintained but may be unavailable for consistent detection in peripheral blood, and that these antigens induce both pro-inflammatory and anti-inflammatory cytokine responses in this population.

UR - http://www.scopus.com/inward/record.url?scp=68949098057&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=68949098057&partnerID=8YFLogxK

M3 - Article

VL - 81

SP - 489

EP - 495

JO - American Journal of Tropical Medicine and Hygiene

JF - American Journal of Tropical Medicine and Hygiene

SN - 0002-9637

IS - 3

ER -