Stability of the δ-endotoxin gene from Bacillus thuringiensis subsp. kurstaki in a recombinant strain of Clavibacter xyli subsp. cynodontis

J. T. Turner, J. S. Lampel, Robert Stearman, G. W. Sundin, P. Gunyuzlu, J. J. Anderson

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

Deletion of chromosomally inserted gene sequences from Clavibacter xyli subsp. cynodontis, a xylem-inhabiting endophyte, was studied in vitro and in planta. We found that nonreplicating plasmid pCG610, which conferred resistance to kanamycin and tetracycline and contained segments of C. xyli subsp. cynodontis genomic DNA, integrated into a homologous sequence in the bacterial chromosome. In addition, pCG610 contains two copies of the gene encoding the CryIA(c) insecticidal protein of Bacillus thuringiensis subsp. kurstaki HD73. Using drug resistance phenotypes and specific DNA probes, we found that the loss of all three genes arose both in vitro under nonselective conditions and in planta. The resulting segregants are probably formed by recombination between the repeated DNA sequences flanking pCG610 that resulted from the integration event into the chromosome. Eventually, segregants predominated in the bacterial population. The loss of the integrated plasmid from C. xyli subsp. cynodontis revealed a possible approach for decreasing the environmental consequences of recombinant bacteria for agricultural use.

Original languageEnglish (US)
Pages (from-to)3522-3528
Number of pages7
JournalApplied and Environmental Microbiology
Volume57
Issue number12
StatePublished - 1991
Externally publishedYes

Fingerprint

Leifsonia xyli subsp. cynodontis
Bacillus thuringiensis subsp. kurstaki
Bacillus thuringiensis
endotoxins
Endotoxins
Plantae
plasmid
DNA
gene
chromosome
plasmids
Plasmids
Bacterial Chromosomes
Kanamycin Resistance
Genes
Endophytes
chromosomes
drug resistance
Tetracycline Resistance
nucleotide sequences

ASJC Scopus subject areas

  • Biotechnology
  • Environmental Science(all)
  • Microbiology

Cite this

Stability of the δ-endotoxin gene from Bacillus thuringiensis subsp. kurstaki in a recombinant strain of Clavibacter xyli subsp. cynodontis. / Turner, J. T.; Lampel, J. S.; Stearman, Robert; Sundin, G. W.; Gunyuzlu, P.; Anderson, J. J.

In: Applied and Environmental Microbiology, Vol. 57, No. 12, 1991, p. 3522-3528.

Research output: Contribution to journalArticle

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