Stability of the infection marker struvite in urinary stone samples

James Williams, Andrew J. Sacks, Kate Englert, Rachel Deal, Takeisha L. Farmer, Molly E. Jackson, James E. Lingeman, James A. McAteer

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Background and Purpose: Struvite in kidney stones is an important marker for infection. In kidney stone samples, struvite is known to be prone to chemical breakdown, but no data exist on the stability of samples stored in dry form. The objective of this study was to examine stability of struvite under increasingly poor conditions of storage. Materials and Methods: Samples of struvite kidney stones were broken to obtain 38 pieces averaging 67 mg in weight, and these were randomized into four storage conditions: Airtight containers stored in the dark, open containers in the dark, open containers in ambient light, and open containers at elevated temperature (40°C). Pieces were left for 6 months, and then analyzed for changes using micro CT and Fourier transform infrared spectroscopy (FT-IR). Results: Initial samples proved to be struvite, indicating no transformation in the large specimens that had been stored in airtight containers in the dark for more than 6 years before this study. Pieces of struvite taken from these large specimens appeared unchanged by micro CT and FT-IR after being stored in closed containers for 6 months, but 8 of 9 pieces in open containers showed the presence of newberyite in surface layers, as did 10 of 10 pieces in open containers out in ambient light. All pieces stored at 40°C showed transformation of struvite, with 60% of the pieces showing the presence of amorphous phosphates, indicating complete breakdown of struvite in the surface layers of the pieces. Conclusion: We conclude that struvite in dry kidney stone samples is stable when the specimens are stored in airtight containers at room temperature, even after several years.

Original languageEnglish
Pages (from-to)726-731
Number of pages6
JournalJournal of Endourology
Volume26
Issue number6
DOIs
StatePublished - Jun 1 2012

Fingerprint

Urinary Calculi
Kidney Calculi
Infection
Fourier Transform Infrared Spectroscopy
Struvite
Light
Temperature
Phosphates
Weights and Measures

ASJC Scopus subject areas

  • Urology

Cite this

Williams, J., Sacks, A. J., Englert, K., Deal, R., Farmer, T. L., Jackson, M. E., ... McAteer, J. A. (2012). Stability of the infection marker struvite in urinary stone samples. Journal of Endourology, 26(6), 726-731. https://doi.org/10.1089/end.2011.0274

Stability of the infection marker struvite in urinary stone samples. / Williams, James; Sacks, Andrew J.; Englert, Kate; Deal, Rachel; Farmer, Takeisha L.; Jackson, Molly E.; Lingeman, James E.; McAteer, James A.

In: Journal of Endourology, Vol. 26, No. 6, 01.06.2012, p. 726-731.

Research output: Contribution to journalArticle

Williams, J, Sacks, AJ, Englert, K, Deal, R, Farmer, TL, Jackson, ME, Lingeman, JE & McAteer, JA 2012, 'Stability of the infection marker struvite in urinary stone samples', Journal of Endourology, vol. 26, no. 6, pp. 726-731. https://doi.org/10.1089/end.2011.0274
Williams, James ; Sacks, Andrew J. ; Englert, Kate ; Deal, Rachel ; Farmer, Takeisha L. ; Jackson, Molly E. ; Lingeman, James E. ; McAteer, James A. / Stability of the infection marker struvite in urinary stone samples. In: Journal of Endourology. 2012 ; Vol. 26, No. 6. pp. 726-731.
@article{0da2435034484c979b73e3fc484951d5,
title = "Stability of the infection marker struvite in urinary stone samples",
abstract = "Background and Purpose: Struvite in kidney stones is an important marker for infection. In kidney stone samples, struvite is known to be prone to chemical breakdown, but no data exist on the stability of samples stored in dry form. The objective of this study was to examine stability of struvite under increasingly poor conditions of storage. Materials and Methods: Samples of struvite kidney stones were broken to obtain 38 pieces averaging 67 mg in weight, and these were randomized into four storage conditions: Airtight containers stored in the dark, open containers in the dark, open containers in ambient light, and open containers at elevated temperature (40°C). Pieces were left for 6 months, and then analyzed for changes using micro CT and Fourier transform infrared spectroscopy (FT-IR). Results: Initial samples proved to be struvite, indicating no transformation in the large specimens that had been stored in airtight containers in the dark for more than 6 years before this study. Pieces of struvite taken from these large specimens appeared unchanged by micro CT and FT-IR after being stored in closed containers for 6 months, but 8 of 9 pieces in open containers showed the presence of newberyite in surface layers, as did 10 of 10 pieces in open containers out in ambient light. All pieces stored at 40°C showed transformation of struvite, with 60{\%} of the pieces showing the presence of amorphous phosphates, indicating complete breakdown of struvite in the surface layers of the pieces. Conclusion: We conclude that struvite in dry kidney stone samples is stable when the specimens are stored in airtight containers at room temperature, even after several years.",
author = "James Williams and Sacks, {Andrew J.} and Kate Englert and Rachel Deal and Farmer, {Takeisha L.} and Jackson, {Molly E.} and Lingeman, {James E.} and McAteer, {James A.}",
year = "2012",
month = "6",
day = "1",
doi = "10.1089/end.2011.0274",
language = "English",
volume = "26",
pages = "726--731",
journal = "Journal of Endourology",
issn = "0892-7790",
publisher = "Mary Ann Liebert Inc.",
number = "6",

}

TY - JOUR

T1 - Stability of the infection marker struvite in urinary stone samples

AU - Williams, James

AU - Sacks, Andrew J.

AU - Englert, Kate

AU - Deal, Rachel

AU - Farmer, Takeisha L.

AU - Jackson, Molly E.

AU - Lingeman, James E.

AU - McAteer, James A.

PY - 2012/6/1

Y1 - 2012/6/1

N2 - Background and Purpose: Struvite in kidney stones is an important marker for infection. In kidney stone samples, struvite is known to be prone to chemical breakdown, but no data exist on the stability of samples stored in dry form. The objective of this study was to examine stability of struvite under increasingly poor conditions of storage. Materials and Methods: Samples of struvite kidney stones were broken to obtain 38 pieces averaging 67 mg in weight, and these were randomized into four storage conditions: Airtight containers stored in the dark, open containers in the dark, open containers in ambient light, and open containers at elevated temperature (40°C). Pieces were left for 6 months, and then analyzed for changes using micro CT and Fourier transform infrared spectroscopy (FT-IR). Results: Initial samples proved to be struvite, indicating no transformation in the large specimens that had been stored in airtight containers in the dark for more than 6 years before this study. Pieces of struvite taken from these large specimens appeared unchanged by micro CT and FT-IR after being stored in closed containers for 6 months, but 8 of 9 pieces in open containers showed the presence of newberyite in surface layers, as did 10 of 10 pieces in open containers out in ambient light. All pieces stored at 40°C showed transformation of struvite, with 60% of the pieces showing the presence of amorphous phosphates, indicating complete breakdown of struvite in the surface layers of the pieces. Conclusion: We conclude that struvite in dry kidney stone samples is stable when the specimens are stored in airtight containers at room temperature, even after several years.

AB - Background and Purpose: Struvite in kidney stones is an important marker for infection. In kidney stone samples, struvite is known to be prone to chemical breakdown, but no data exist on the stability of samples stored in dry form. The objective of this study was to examine stability of struvite under increasingly poor conditions of storage. Materials and Methods: Samples of struvite kidney stones were broken to obtain 38 pieces averaging 67 mg in weight, and these were randomized into four storage conditions: Airtight containers stored in the dark, open containers in the dark, open containers in ambient light, and open containers at elevated temperature (40°C). Pieces were left for 6 months, and then analyzed for changes using micro CT and Fourier transform infrared spectroscopy (FT-IR). Results: Initial samples proved to be struvite, indicating no transformation in the large specimens that had been stored in airtight containers in the dark for more than 6 years before this study. Pieces of struvite taken from these large specimens appeared unchanged by micro CT and FT-IR after being stored in closed containers for 6 months, but 8 of 9 pieces in open containers showed the presence of newberyite in surface layers, as did 10 of 10 pieces in open containers out in ambient light. All pieces stored at 40°C showed transformation of struvite, with 60% of the pieces showing the presence of amorphous phosphates, indicating complete breakdown of struvite in the surface layers of the pieces. Conclusion: We conclude that struvite in dry kidney stone samples is stable when the specimens are stored in airtight containers at room temperature, even after several years.

UR - http://www.scopus.com/inward/record.url?scp=84861976860&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84861976860&partnerID=8YFLogxK

U2 - 10.1089/end.2011.0274

DO - 10.1089/end.2011.0274

M3 - Article

C2 - 21988101

AN - SCOPUS:84861976860

VL - 26

SP - 726

EP - 731

JO - Journal of Endourology

JF - Journal of Endourology

SN - 0892-7790

IS - 6

ER -