Standardization of the experimental model of Haemophilus ducreyi infection in human subjects

Jaffar A. Al-Tawfiq, Alice C. Thornton, Barry P. Katz, Kate R. Fortney, Kristin D. Todd, Antoinette F. Hood, Stanley M. Spinola

Research output: Contribution to journalArticle

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Abstract

Human volunteers were challenged with Haemophilus ducreyi. Twenty subjects were inoculated with 2 doses (~30 cfu) of live and I dose of heat- killed bacteria at 3 sites on the arm. Eight subjectS were assigned to biopsy 1 or 4 days after inoculation, and 12 were biopsied after they developed a painful pustular lesion or were followed until disease resolved. Papules developed at 95% of 40 sites infected with live bacteria (95% confidence interval [CI], 83.1%-99.4%). In 24 sites followed to end point, 27% of the papules resolved, 69% (95% CI, 47.1%-86.6%) evolved into pustules, and 4% remained at the papular stage. Recovery rates of H. ducreyi from surface cultures ranged from 13% to 41%. H. ducreyi was recovered from biopsies of 12 of 15 pustules and 1 of 7 papules, suggesting that H. ducreyi replicates between the papular and pustular stages of disease.

Original languageEnglish
Pages (from-to)1684-1687
Number of pages4
JournalJournal of Infectious Diseases
Volume178
Issue number6
DOIs
StatePublished - 1998

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Haemophilus ducreyi
Haemophilus Infections
Theoretical Models
Confidence Intervals
Bacteria
Biopsy
Volunteers
Hot Temperature

ASJC Scopus subject areas

  • Public Health, Environmental and Occupational Health
  • Immunology

Cite this

Standardization of the experimental model of Haemophilus ducreyi infection in human subjects. / Al-Tawfiq, Jaffar A.; Thornton, Alice C.; Katz, Barry P.; Fortney, Kate R.; Todd, Kristin D.; Hood, Antoinette F.; Spinola, Stanley M.

In: Journal of Infectious Diseases, Vol. 178, No. 6, 1998, p. 1684-1687.

Research output: Contribution to journalArticle

Al-Tawfiq, Jaffar A. ; Thornton, Alice C. ; Katz, Barry P. ; Fortney, Kate R. ; Todd, Kristin D. ; Hood, Antoinette F. ; Spinola, Stanley M. / Standardization of the experimental model of Haemophilus ducreyi infection in human subjects. In: Journal of Infectious Diseases. 1998 ; Vol. 178, No. 6. pp. 1684-1687.
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