Staphylococcal lipoteichoic acid inhibits delayed-type hypersensitivity reactions via the platelet-activating factor receptor

Qiwei Zhang, Nico Mousdicas, Qiaofang Yi, Mohammed Al-Hassani, Steven D. Billings, Susan Perkins, Katherine M. Howard, Satoshi Ishii, Takao Shimizu, Jeffrey Travers

Research output: Contribution to journalArticle

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Abstract

Staphylococcus aureus infections are known triggers for skin inflammation and can modulate immune responses. The present studies used model systems consisting of platelet-activating factor receptor-positive and -negative (PAF-R-positive and -negative) cells and PAF-R-deficient mice to demonstrate that staphylococcal lipoteichoic acid (LTA), a constituent of Gram-positive bacteria cell walls, acts as a PAF-R agonist. We show that LTA stimulates an immediate intracellular Ca2+ flux only in PAF-R-positive cells. Intradermal injections of LTA and the PAF-R agonist 1-hexadecyl-2-N- methylcarbamoyl glycerophosphocholine (CPAF) induced cutaneous inflammation in wild-type but not PAF-R-deficient mice. Systemic exposure to LTA or CPAF inhibited delayed-type hypersensitivity (DTH) reactions to the chemical dinitrofluorobenzene only in PAF-R-expressing mice. The inhibition of DTH reactions was abrogated by the addition of neutralizing antibodies to IL-10. Finally, we measured levels of LTA that were adequate to stimulate PAF-R in vitro on the skin of subjects with infected atopic dermatitis. Based on these studies, we propose that LTA exerts immunomodulatory effects via the PAF-R through production of the Th2 cytokine IL-10. These findings show a novel mechanism by which staphylococcal infections can inhibit Th1 reactions and thus worsen Th2 skin diseases, such as atopic dermatitis.

Original languageEnglish
Pages (from-to)2855-2861
Number of pages7
JournalJournal of Clinical Investigation
Volume115
Issue number10
DOIs
StatePublished - Oct 2005

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Delayed Hypersensitivity
Atopic Dermatitis
Interleukin-10
Skin
Dinitrofluorobenzene
Inflammation
Staphylococcal Infections
Intradermal Injections
Gram-Positive Bacteria
Neutralizing Antibodies
Skin Diseases
Cell Wall
Staphylococcus aureus
platelet activating factor receptor
lipoteichoic acid
Cytokines
Infection

ASJC Scopus subject areas

  • Medicine(all)

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Staphylococcal lipoteichoic acid inhibits delayed-type hypersensitivity reactions via the platelet-activating factor receptor. / Zhang, Qiwei; Mousdicas, Nico; Yi, Qiaofang; Al-Hassani, Mohammed; Billings, Steven D.; Perkins, Susan; Howard, Katherine M.; Ishii, Satoshi; Shimizu, Takao; Travers, Jeffrey.

In: Journal of Clinical Investigation, Vol. 115, No. 10, 10.2005, p. 2855-2861.

Research output: Contribution to journalArticle

Zhang, Qiwei ; Mousdicas, Nico ; Yi, Qiaofang ; Al-Hassani, Mohammed ; Billings, Steven D. ; Perkins, Susan ; Howard, Katherine M. ; Ishii, Satoshi ; Shimizu, Takao ; Travers, Jeffrey. / Staphylococcal lipoteichoic acid inhibits delayed-type hypersensitivity reactions via the platelet-activating factor receptor. In: Journal of Clinical Investigation. 2005 ; Vol. 115, No. 10. pp. 2855-2861.
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