TY - JOUR
T1 - Staphylococcus aureus peptidoglycan is a toll-like receptor 2 activator
T2 - A reevaluation
AU - Dziarski, Roman
AU - Gupta, Dipika
PY - 2005/8/1
Y1 - 2005/8/1
N2 - Since the ability of peptidoglycan (PGN) to activate Toll-like receptor 2 (TLR2) was recently questioned, we reevaluated activation of TLR2 by PGN. Polymeric soluble or insoluble Staphylococcus aureus PGN, repurified by sodium dodecyl sulfate or phenol extraction, activated TLR2 at 0.1 to 1 or 10 μg/ml, respectively, and induced tumor necrosis factor alpha production. The TLR2 activation by PGN, but not by lipoteichoic acid, was abolished by muramidase digestion. We conclude that polymeric S. aureus PGN is a TLR2 activator.
AB - Since the ability of peptidoglycan (PGN) to activate Toll-like receptor 2 (TLR2) was recently questioned, we reevaluated activation of TLR2 by PGN. Polymeric soluble or insoluble Staphylococcus aureus PGN, repurified by sodium dodecyl sulfate or phenol extraction, activated TLR2 at 0.1 to 1 or 10 μg/ml, respectively, and induced tumor necrosis factor alpha production. The TLR2 activation by PGN, but not by lipoteichoic acid, was abolished by muramidase digestion. We conclude that polymeric S. aureus PGN is a TLR2 activator.
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U2 - 10.1128/IAI.73.8.5212-5216.2005
DO - 10.1128/IAI.73.8.5212-5216.2005
M3 - Article
C2 - 16041042
AN - SCOPUS:23344449406
VL - 73
SP - 5212
EP - 5216
JO - Infection and Immunity
JF - Infection and Immunity
SN - 0019-9567
IS - 8
ER -