Abstract
Since the ability of peptidoglycan (PGN) to activate Toll-like receptor 2 (TLR2) was recently questioned, we reevaluated activation of TLR2 by PGN. Polymeric soluble or insoluble Staphylococcus aureus PGN, repurified by sodium dodecyl sulfate or phenol extraction, activated TLR2 at 0.1 to 1 or 10 μg/ml, respectively, and induced tumor necrosis factor alpha production. The TLR2 activation by PGN, but not by lipoteichoic acid, was abolished by muramidase digestion. We conclude that polymeric S. aureus PGN is a TLR2 activator.
| Original language | English |
|---|---|
| Pages (from-to) | 5212-5216 |
| Number of pages | 5 |
| Journal | Infection and Immunity |
| Volume | 73 |
| Issue number | 8 |
| DOIs | |
| State | Published - Aug 2005 |
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ASJC Scopus subject areas
- Immunology
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Staphylococcus aureus peptidoglycan is a toll-like receptor 2 activator : A reevaluation. / Dziarski, Roman; Gupta, Dipika.
In: Infection and Immunity, Vol. 73, No. 8, 08.2005, p. 5212-5216.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Staphylococcus aureus peptidoglycan is a toll-like receptor 2 activator
T2 - A reevaluation
AU - Dziarski, Roman
AU - Gupta, Dipika
PY - 2005/8
Y1 - 2005/8
N2 - Since the ability of peptidoglycan (PGN) to activate Toll-like receptor 2 (TLR2) was recently questioned, we reevaluated activation of TLR2 by PGN. Polymeric soluble or insoluble Staphylococcus aureus PGN, repurified by sodium dodecyl sulfate or phenol extraction, activated TLR2 at 0.1 to 1 or 10 μg/ml, respectively, and induced tumor necrosis factor alpha production. The TLR2 activation by PGN, but not by lipoteichoic acid, was abolished by muramidase digestion. We conclude that polymeric S. aureus PGN is a TLR2 activator.
AB - Since the ability of peptidoglycan (PGN) to activate Toll-like receptor 2 (TLR2) was recently questioned, we reevaluated activation of TLR2 by PGN. Polymeric soluble or insoluble Staphylococcus aureus PGN, repurified by sodium dodecyl sulfate or phenol extraction, activated TLR2 at 0.1 to 1 or 10 μg/ml, respectively, and induced tumor necrosis factor alpha production. The TLR2 activation by PGN, but not by lipoteichoic acid, was abolished by muramidase digestion. We conclude that polymeric S. aureus PGN is a TLR2 activator.
UR - http://www.scopus.com/inward/record.url?scp=23344449406&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=23344449406&partnerID=8YFLogxK
U2 - 10.1128/IAI.73.8.5212-5216.2005
DO - 10.1128/IAI.73.8.5212-5216.2005
M3 - Article
C2 - 16041042
AN - SCOPUS:23344449406
VL - 73
SP - 5212
EP - 5216
JO - Infection and Immunity
JF - Infection and Immunity
SN - 0019-9567
IS - 8
ER -