Staphylococcus aureus virulence genes identified by bursa aurealis mutagenesis and nematode killing

Taeok Bae, Alison K. Banger, Adam Wallace, Elizabeth M. Glass, Fredrik Åslund, Olaf Schneewind, Dominique M. Missiakas

Research output: Contribution to journalArticle

223 Scopus citations

Abstract

Staphylococcus aureus is the leading cause of wound and hospital-acquired infections worldwide. The emergence of S. aureus strains with resistance to multiple antibiotics requires the identification of bacterial virulence genes and the development of novel therapeutic strategies. Herein, bursa aurealis, a mariner-based transposon, was used for random mutagenesis and for the isolation of 10,325 S. aureus variants with defined insertion sites. By screening for loss-of-function mutants in a Caenorhabditis elegans killing assay, 71 S. aureus virulence genes were identified. Some of these genes are also required for S. aureus abscess formation in a murine infection model.

Original languageEnglish (US)
Pages (from-to)12312-12317
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume101
Issue number33
DOIs
StatePublished - Aug 17 2004
Externally publishedYes

Keywords

  • Caenorhabditis elegans
  • Transposon

ASJC Scopus subject areas

  • General

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