Staphylococcus-induced urolithiasis in estrogen-treated ovariectomized nude mice

Lona K. Gibbs, Debra Hickman, Anne D. Lewis, Lois M A Colgin

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Nine of 24 ovariectomized nude mice developed ulcerative skin lesions 28 d after implantation with human breast cancer cells and slow-release estrogen pellets. Aerobic culture of samples from the skin lesions yielded Staphylococcus intermedius. By day 45 postimplantation, all mice displayed ulcerative skin lesions, and 5 mice exhibited hunched posture, listless behavior, cyanosis, anorexia, and dehydration. This subset was euthanized but not necropsied. When additional animals became moribund, the investigator elected to end the study at day 51. At necropsy, all 20 but 1 had cystitis and urolithiasis, characterized by relatively large struvite stones and crystals that had filled the urinary bladders of the research animals and caused severe thickening of the bladder walls. Gram-positive cocci were observed microscopically in both the kidneys and bladders of the necropsied animals. Spontaneous urolithiasis has not previously been documented to occur in association with infection of female nude mice by S. intermedius.

Original languageEnglish (US)
Pages (from-to)61-65
Number of pages5
JournalJournal of the American Association for Laboratory Animal Science
Volume46
Issue number4
StatePublished - Jul 2007
Externally publishedYes

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Staphylococcus
estrogens
skin lesions
bladder
Staphylococcus intermedius
mice
magnesium ammonium phosphate
cystitis
dehydration (animal physiology)
Coccus
posture
anorexia
breast neoplasms
laboratory animals
crystals
pellets
necropsy
animals
kidneys
urolithiasis

ASJC Scopus subject areas

  • Animal Science and Zoology

Cite this

Staphylococcus-induced urolithiasis in estrogen-treated ovariectomized nude mice. / Gibbs, Lona K.; Hickman, Debra; Lewis, Anne D.; Colgin, Lois M A.

In: Journal of the American Association for Laboratory Animal Science, Vol. 46, No. 4, 07.2007, p. 61-65.

Research output: Contribution to journalArticle

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