Starvation and diabetes increase the amount of pyruvate dehydrogenase kinase isoenzyme 4 in rat heart

Pengfei Wu, Juichi Sato, Yu Zhao, Jerzy Jaskiewicz, Kirill M. Popov, Robert Harris

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Abstract

This study investigated whether conditions known to alter the activity and phosphorylation state of the pyruvate dehydrogenase complex have specific effects on the levels of isoenzymes of pyruvate dehydrogenase kinase (PDK) in rat heart. Immunoblot analysis revealed a remarkable increase in the amount of PDK4 in the hearts of rats that had been starved or rendered diabetic with streptozotocin. Re-feeding of starved rats and insulin treatment of diabetic rats very effectively reversed the increase in PDK4 protein and restored PDK enzyme activity to levels of chow-fed control rats. Starvation and diabetes also markedly increased the abundance of PDK4 mRNA, and re-feeding and insulin treatment reduced levels of the message to that of controls. In contrast with the findings for PDK4, little or no changes in the amounts of PDK1 and PDK2 protein and the abundance of their messages occurred in response to starvation and diabetes. The observed shift in the relative abundance of PDK isoenzymes probably explains previous studies of the effects of starvation and diabetes on heart PDK activity. The results indicate that control of the amount of PDK4 is important in long-term regulation of the activity of the pyruvate dehydrogenase complex in rat heart.

Original languageEnglish
Pages (from-to)197-201
Number of pages5
JournalBiochemical Journal
Volume329
Issue number1
StatePublished - Jan 1 1998

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Medical problems
Starvation
Isoenzymes
Rats
Pyruvate Dehydrogenase Complex
Rat control
Insulin
Phosphorylation
Enzyme activity
Streptozocin
Proteins
pyruvate dehydrogenase (acetyl-transferring) kinase
Messenger RNA
Enzymes

ASJC Scopus subject areas

  • Biochemistry

Cite this

Starvation and diabetes increase the amount of pyruvate dehydrogenase kinase isoenzyme 4 in rat heart. / Wu, Pengfei; Sato, Juichi; Zhao, Yu; Jaskiewicz, Jerzy; Popov, Kirill M.; Harris, Robert.

In: Biochemical Journal, Vol. 329, No. 1, 01.01.1998, p. 197-201.

Research output: Contribution to journalArticle

Wu, P, Sato, J, Zhao, Y, Jaskiewicz, J, Popov, KM & Harris, R 1998, 'Starvation and diabetes increase the amount of pyruvate dehydrogenase kinase isoenzyme 4 in rat heart', Biochemical Journal, vol. 329, no. 1, pp. 197-201.
Wu, Pengfei ; Sato, Juichi ; Zhao, Yu ; Jaskiewicz, Jerzy ; Popov, Kirill M. ; Harris, Robert. / Starvation and diabetes increase the amount of pyruvate dehydrogenase kinase isoenzyme 4 in rat heart. In: Biochemical Journal. 1998 ; Vol. 329, No. 1. pp. 197-201.
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