Stat3 Is Important for Follicular Regulatory T Cell Differentiation

Hao Wu, Markus M. Xie, Hong Liu, Alexander Dent

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

The production of antibody is precisely controlled during the germinal center (GC) reaction. This process is dependent on the help from follicular T helper (Tfh) cells to germinal center (GC) B cells and is regulated by regulatory follicular T helper (Tfr) cells. How Tfr cells develop and how their suppressive activity functions are not well understood. Here, we found that Stat3 is indispensible for Tfr cell differentiation. After immunization with Sheep Red Blood Cells (SRBC), the loss of Tfr cells caused by deletion of Stat3 in Treg cells does not affect the size of Tfh or GC B cell population, but rather leads to strongly enhanced production of antigen-specific IgG1 and IgG2b. In Peyer's patches (PPs) in the gut, we found that Stat3 expression in Treg cells is also required for Tfr cell formation to commensal organisms. However, loss of Tfr cells in the gut did not affect the numbers of Tfh cells and GC B cells, nor affect IgG1 or IgA switching by GC B cells. Overall, our study has uncovered unique roles of Stat3 in Tfr cell differentiation and the regulation of the antibody response.

Original languageEnglish (US)
Pages (from-to)e0155040
JournalPLoS One
Volume11
Issue number5
DOIs
StatePublished - 2016

Fingerprint

Germinal Center
T-cells
Regulatory T-Lymphocytes
cell differentiation
Cell Differentiation
T-lymphocytes
Cells
B-Lymphocytes
Helper-Inducer T-Lymphocytes
B-lymphocytes
Antibody Formation
cells
Immunoglobulin G
Immunization
Peyer's Patches
Antibodies
Immunoglobulin A
digestive system
Sheep
Blood

ASJC Scopus subject areas

  • Medicine(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Stat3 Is Important for Follicular Regulatory T Cell Differentiation. / Wu, Hao; Xie, Markus M.; Liu, Hong; Dent, Alexander.

In: PLoS One, Vol. 11, No. 5, 2016, p. e0155040.

Research output: Contribution to journalArticle

Wu, Hao ; Xie, Markus M. ; Liu, Hong ; Dent, Alexander. / Stat3 Is Important for Follicular Regulatory T Cell Differentiation. In: PLoS One. 2016 ; Vol. 11, No. 5. pp. e0155040.
@article{ffd257cbb1094a6aa928282089ae4b50,
title = "Stat3 Is Important for Follicular Regulatory T Cell Differentiation",
abstract = "The production of antibody is precisely controlled during the germinal center (GC) reaction. This process is dependent on the help from follicular T helper (Tfh) cells to germinal center (GC) B cells and is regulated by regulatory follicular T helper (Tfr) cells. How Tfr cells develop and how their suppressive activity functions are not well understood. Here, we found that Stat3 is indispensible for Tfr cell differentiation. After immunization with Sheep Red Blood Cells (SRBC), the loss of Tfr cells caused by deletion of Stat3 in Treg cells does not affect the size of Tfh or GC B cell population, but rather leads to strongly enhanced production of antigen-specific IgG1 and IgG2b. In Peyer's patches (PPs) in the gut, we found that Stat3 expression in Treg cells is also required for Tfr cell formation to commensal organisms. However, loss of Tfr cells in the gut did not affect the numbers of Tfh cells and GC B cells, nor affect IgG1 or IgA switching by GC B cells. Overall, our study has uncovered unique roles of Stat3 in Tfr cell differentiation and the regulation of the antibody response.",
author = "Hao Wu and Xie, {Markus M.} and Hong Liu and Alexander Dent",
year = "2016",
doi = "10.1371/journal.pone.0155040",
language = "English (US)",
volume = "11",
pages = "e0155040",
journal = "PLoS One",
issn = "1932-6203",
publisher = "Public Library of Science",
number = "5",

}

TY - JOUR

T1 - Stat3 Is Important for Follicular Regulatory T Cell Differentiation

AU - Wu, Hao

AU - Xie, Markus M.

AU - Liu, Hong

AU - Dent, Alexander

PY - 2016

Y1 - 2016

N2 - The production of antibody is precisely controlled during the germinal center (GC) reaction. This process is dependent on the help from follicular T helper (Tfh) cells to germinal center (GC) B cells and is regulated by regulatory follicular T helper (Tfr) cells. How Tfr cells develop and how their suppressive activity functions are not well understood. Here, we found that Stat3 is indispensible for Tfr cell differentiation. After immunization with Sheep Red Blood Cells (SRBC), the loss of Tfr cells caused by deletion of Stat3 in Treg cells does not affect the size of Tfh or GC B cell population, but rather leads to strongly enhanced production of antigen-specific IgG1 and IgG2b. In Peyer's patches (PPs) in the gut, we found that Stat3 expression in Treg cells is also required for Tfr cell formation to commensal organisms. However, loss of Tfr cells in the gut did not affect the numbers of Tfh cells and GC B cells, nor affect IgG1 or IgA switching by GC B cells. Overall, our study has uncovered unique roles of Stat3 in Tfr cell differentiation and the regulation of the antibody response.

AB - The production of antibody is precisely controlled during the germinal center (GC) reaction. This process is dependent on the help from follicular T helper (Tfh) cells to germinal center (GC) B cells and is regulated by regulatory follicular T helper (Tfr) cells. How Tfr cells develop and how their suppressive activity functions are not well understood. Here, we found that Stat3 is indispensible for Tfr cell differentiation. After immunization with Sheep Red Blood Cells (SRBC), the loss of Tfr cells caused by deletion of Stat3 in Treg cells does not affect the size of Tfh or GC B cell population, but rather leads to strongly enhanced production of antigen-specific IgG1 and IgG2b. In Peyer's patches (PPs) in the gut, we found that Stat3 expression in Treg cells is also required for Tfr cell formation to commensal organisms. However, loss of Tfr cells in the gut did not affect the numbers of Tfh cells and GC B cells, nor affect IgG1 or IgA switching by GC B cells. Overall, our study has uncovered unique roles of Stat3 in Tfr cell differentiation and the regulation of the antibody response.

UR - http://www.scopus.com/inward/record.url?scp=85020743755&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85020743755&partnerID=8YFLogxK

U2 - 10.1371/journal.pone.0155040

DO - 10.1371/journal.pone.0155040

M3 - Article

VL - 11

SP - e0155040

JO - PLoS One

JF - PLoS One

SN - 1932-6203

IS - 5

ER -