STAT3 Is Required for Flt3L-Dependent Dendritic Cell Differentiation

Yasmina Laouar, Thomas Welte, Xin Yuan Fu, Richard A. Flavell

Research output: Contribution to journalArticle

222 Citations (Scopus)

Abstract

The signals that control decisions of progenitor commitment involve the interplay of both cytokines and transcription factors. Flt3L has emerged as a potential regulator of dendritic cell (DC) development, but regulation of HSC commitment to the DC lineage remains poorly understood. Our central finding is the identification of STAT3 activation as a checkpoint of Flt3L-regulated DC development. Deletion of STAT3 caused profound deficiency in the DC compartment and abrogated Flt3L effects on DC development. DC derivation by Flt3L revealed a normal HSC pool, a 2- to 3-fold accumulation of CLP/CMP, but absence of common DC precursors as well as their DC progeny in STAT3-deficient mice. The formation of CMP and CLP represents the first decisive lineage commitment step, and in this regard we provide evidence that commitments of CLP/CMP to the DC lineage strictly depend on the interplay of both Flt3L and STAT3 activation.

Original languageEnglish (US)
Pages (from-to)903-912
Number of pages10
JournalImmunity
Volume19
Issue number6
DOIs
StatePublished - Dec 2003
Externally publishedYes

Fingerprint

Dendritic Cells
Cell Differentiation
Cytidine Monophosphate
Cell Lineage
Transcription Factors
Cytokines

ASJC Scopus subject areas

  • Immunology and Allergy
  • Infectious Diseases
  • Immunology

Cite this

STAT3 Is Required for Flt3L-Dependent Dendritic Cell Differentiation. / Laouar, Yasmina; Welte, Thomas; Fu, Xin Yuan; Flavell, Richard A.

In: Immunity, Vol. 19, No. 6, 12.2003, p. 903-912.

Research output: Contribution to journalArticle

Laouar, Y, Welte, T, Fu, XY & Flavell, RA 2003, 'STAT3 Is Required for Flt3L-Dependent Dendritic Cell Differentiation', Immunity, vol. 19, no. 6, pp. 903-912. https://doi.org/10.1016/S1074-7613(03)00332-7
Laouar, Yasmina ; Welte, Thomas ; Fu, Xin Yuan ; Flavell, Richard A. / STAT3 Is Required for Flt3L-Dependent Dendritic Cell Differentiation. In: Immunity. 2003 ; Vol. 19, No. 6. pp. 903-912.
@article{12df9b9bfb16446e8bf628e67795704a,
title = "STAT3 Is Required for Flt3L-Dependent Dendritic Cell Differentiation",
abstract = "The signals that control decisions of progenitor commitment involve the interplay of both cytokines and transcription factors. Flt3L has emerged as a potential regulator of dendritic cell (DC) development, but regulation of HSC commitment to the DC lineage remains poorly understood. Our central finding is the identification of STAT3 activation as a checkpoint of Flt3L-regulated DC development. Deletion of STAT3 caused profound deficiency in the DC compartment and abrogated Flt3L effects on DC development. DC derivation by Flt3L revealed a normal HSC pool, a 2- to 3-fold accumulation of CLP/CMP, but absence of common DC precursors as well as their DC progeny in STAT3-deficient mice. The formation of CMP and CLP represents the first decisive lineage commitment step, and in this regard we provide evidence that commitments of CLP/CMP to the DC lineage strictly depend on the interplay of both Flt3L and STAT3 activation.",
author = "Yasmina Laouar and Thomas Welte and Fu, {Xin Yuan} and Flavell, {Richard A.}",
year = "2003",
month = "12",
doi = "10.1016/S1074-7613(03)00332-7",
language = "English (US)",
volume = "19",
pages = "903--912",
journal = "Immunity",
issn = "1074-7613",
publisher = "Cell Press",
number = "6",

}

TY - JOUR

T1 - STAT3 Is Required for Flt3L-Dependent Dendritic Cell Differentiation

AU - Laouar, Yasmina

AU - Welte, Thomas

AU - Fu, Xin Yuan

AU - Flavell, Richard A.

PY - 2003/12

Y1 - 2003/12

N2 - The signals that control decisions of progenitor commitment involve the interplay of both cytokines and transcription factors. Flt3L has emerged as a potential regulator of dendritic cell (DC) development, but regulation of HSC commitment to the DC lineage remains poorly understood. Our central finding is the identification of STAT3 activation as a checkpoint of Flt3L-regulated DC development. Deletion of STAT3 caused profound deficiency in the DC compartment and abrogated Flt3L effects on DC development. DC derivation by Flt3L revealed a normal HSC pool, a 2- to 3-fold accumulation of CLP/CMP, but absence of common DC precursors as well as their DC progeny in STAT3-deficient mice. The formation of CMP and CLP represents the first decisive lineage commitment step, and in this regard we provide evidence that commitments of CLP/CMP to the DC lineage strictly depend on the interplay of both Flt3L and STAT3 activation.

AB - The signals that control decisions of progenitor commitment involve the interplay of both cytokines and transcription factors. Flt3L has emerged as a potential regulator of dendritic cell (DC) development, but regulation of HSC commitment to the DC lineage remains poorly understood. Our central finding is the identification of STAT3 activation as a checkpoint of Flt3L-regulated DC development. Deletion of STAT3 caused profound deficiency in the DC compartment and abrogated Flt3L effects on DC development. DC derivation by Flt3L revealed a normal HSC pool, a 2- to 3-fold accumulation of CLP/CMP, but absence of common DC precursors as well as their DC progeny in STAT3-deficient mice. The formation of CMP and CLP represents the first decisive lineage commitment step, and in this regard we provide evidence that commitments of CLP/CMP to the DC lineage strictly depend on the interplay of both Flt3L and STAT3 activation.

UR - http://www.scopus.com/inward/record.url?scp=0347480225&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0347480225&partnerID=8YFLogxK

U2 - 10.1016/S1074-7613(03)00332-7

DO - 10.1016/S1074-7613(03)00332-7

M3 - Article

VL - 19

SP - 903

EP - 912

JO - Immunity

JF - Immunity

SN - 1074-7613

IS - 6

ER -