STAT3 sensitizes insulin signaling by negatively regulating glycogen synthase kinase-3β

Akira Moh, Wenjun Zhang, Sidney Yu, Jun Wang, Xuming Xu, Jiliang Li, Xin Yuan Fu

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

OBJECTIVE-Glucose homeostasis is achieved by triggering regulation of glycogen synthesis genes in response to insulin when mammals feed, but the underlying molecular mechanism remains largely unknown. The aim of our study was to examine the role of the signal transducers and activators of transcription 3 (STAT3) in insulin signaling. RESEARCH DESIGN AND METHODS-We generated a strain of mice carrying a targeted disruption of Stat3 gene in the liver (L-Stat3 -/- mice). Hepatocytes of the L-Stat3 -/- mice were isolated to establish cell lines for mechanistic studies. Nuclear translocation and DNA-protein interaction of STAT3 was analyzed with immunofluorescent and chromatin immunopre-cipitation methods, respectively. Levels of glucose, insulin, lep-tin, and glucagon were profiled, and putative downstream molecules of STAT3 were examined in the presence of various stimuli in L-Stat3 -/- and control mice. RESULTS-STAT3 was found to sensitize the insulin signaling through suppression of GSK-3β, a negative regulator of insulin signaling pathway. During feeding, both mRNA and protein levels of GSK-3β decreased in Stat3 f/+ mice, which reflected the need of hepatocytes for insulin to induce glycogen synthesis. In contrast, the L-Stat3 -/- mice lost this control and showed a monophasic increase in the GSK-3β level in response to insulin. Administration of GSK-3β inhibitors lithium chloride and L803-mts restored glucose homeostasis and rescued the glucose intolerance and impaired insulin response in L-Stat3 -/- mice. CONCLUSIONS-These data indicate that STAT3 sensitizes insulin signaling by negatively regulating GSK-3β. Inactivation of STAT3 in the liver contributes significantly to the pathogenesis of insulin resistance.

Original languageEnglish
Pages (from-to)1227-1235
Number of pages9
JournalDiabetes
Volume57
Issue number5
DOIs
StatePublished - May 2008

Fingerprint

Glycogen Synthase Kinase 3
STAT3 Transcription Factor
Insulin
Glycogen
Glucose
Hepatocytes
Homeostasis
Lithium Chloride
Glucose Intolerance
Tin
Liver
Protein Transport
Glucagon
Genes
Chromatin
Insulin Resistance
Mammals
Research Design
Cell Line
Messenger RNA

ASJC Scopus subject areas

  • Internal Medicine
  • Endocrinology, Diabetes and Metabolism

Cite this

Moh, A., Zhang, W., Yu, S., Wang, J., Xu, X., Li, J., & Fu, X. Y. (2008). STAT3 sensitizes insulin signaling by negatively regulating glycogen synthase kinase-3β. Diabetes, 57(5), 1227-1235. https://doi.org/10.2337/db06-1582

STAT3 sensitizes insulin signaling by negatively regulating glycogen synthase kinase-3β. / Moh, Akira; Zhang, Wenjun; Yu, Sidney; Wang, Jun; Xu, Xuming; Li, Jiliang; Fu, Xin Yuan.

In: Diabetes, Vol. 57, No. 5, 05.2008, p. 1227-1235.

Research output: Contribution to journalArticle

Moh, A, Zhang, W, Yu, S, Wang, J, Xu, X, Li, J & Fu, XY 2008, 'STAT3 sensitizes insulin signaling by negatively regulating glycogen synthase kinase-3β', Diabetes, vol. 57, no. 5, pp. 1227-1235. https://doi.org/10.2337/db06-1582
Moh, Akira ; Zhang, Wenjun ; Yu, Sidney ; Wang, Jun ; Xu, Xuming ; Li, Jiliang ; Fu, Xin Yuan. / STAT3 sensitizes insulin signaling by negatively regulating glycogen synthase kinase-3β. In: Diabetes. 2008 ; Vol. 57, No. 5. pp. 1227-1235.
@article{a14b1cdbc95740e886395a9c3ee7abb4,
title = "STAT3 sensitizes insulin signaling by negatively regulating glycogen synthase kinase-3β",
abstract = "OBJECTIVE-Glucose homeostasis is achieved by triggering regulation of glycogen synthesis genes in response to insulin when mammals feed, but the underlying molecular mechanism remains largely unknown. The aim of our study was to examine the role of the signal transducers and activators of transcription 3 (STAT3) in insulin signaling. RESEARCH DESIGN AND METHODS-We generated a strain of mice carrying a targeted disruption of Stat3 gene in the liver (L-Stat3 -/- mice). Hepatocytes of the L-Stat3 -/- mice were isolated to establish cell lines for mechanistic studies. Nuclear translocation and DNA-protein interaction of STAT3 was analyzed with immunofluorescent and chromatin immunopre-cipitation methods, respectively. Levels of glucose, insulin, lep-tin, and glucagon were profiled, and putative downstream molecules of STAT3 were examined in the presence of various stimuli in L-Stat3 -/- and control mice. RESULTS-STAT3 was found to sensitize the insulin signaling through suppression of GSK-3β, a negative regulator of insulin signaling pathway. During feeding, both mRNA and protein levels of GSK-3β decreased in Stat3 f/+ mice, which reflected the need of hepatocytes for insulin to induce glycogen synthesis. In contrast, the L-Stat3 -/- mice lost this control and showed a monophasic increase in the GSK-3β level in response to insulin. Administration of GSK-3β inhibitors lithium chloride and L803-mts restored glucose homeostasis and rescued the glucose intolerance and impaired insulin response in L-Stat3 -/- mice. CONCLUSIONS-These data indicate that STAT3 sensitizes insulin signaling by negatively regulating GSK-3β. Inactivation of STAT3 in the liver contributes significantly to the pathogenesis of insulin resistance.",
author = "Akira Moh and Wenjun Zhang and Sidney Yu and Jun Wang and Xuming Xu and Jiliang Li and Fu, {Xin Yuan}",
year = "2008",
month = "5",
doi = "10.2337/db06-1582",
language = "English",
volume = "57",
pages = "1227--1235",
journal = "Diabetes",
issn = "0012-1797",
publisher = "American Diabetes Association Inc.",
number = "5",

}

TY - JOUR

T1 - STAT3 sensitizes insulin signaling by negatively regulating glycogen synthase kinase-3β

AU - Moh, Akira

AU - Zhang, Wenjun

AU - Yu, Sidney

AU - Wang, Jun

AU - Xu, Xuming

AU - Li, Jiliang

AU - Fu, Xin Yuan

PY - 2008/5

Y1 - 2008/5

N2 - OBJECTIVE-Glucose homeostasis is achieved by triggering regulation of glycogen synthesis genes in response to insulin when mammals feed, but the underlying molecular mechanism remains largely unknown. The aim of our study was to examine the role of the signal transducers and activators of transcription 3 (STAT3) in insulin signaling. RESEARCH DESIGN AND METHODS-We generated a strain of mice carrying a targeted disruption of Stat3 gene in the liver (L-Stat3 -/- mice). Hepatocytes of the L-Stat3 -/- mice were isolated to establish cell lines for mechanistic studies. Nuclear translocation and DNA-protein interaction of STAT3 was analyzed with immunofluorescent and chromatin immunopre-cipitation methods, respectively. Levels of glucose, insulin, lep-tin, and glucagon were profiled, and putative downstream molecules of STAT3 were examined in the presence of various stimuli in L-Stat3 -/- and control mice. RESULTS-STAT3 was found to sensitize the insulin signaling through suppression of GSK-3β, a negative regulator of insulin signaling pathway. During feeding, both mRNA and protein levels of GSK-3β decreased in Stat3 f/+ mice, which reflected the need of hepatocytes for insulin to induce glycogen synthesis. In contrast, the L-Stat3 -/- mice lost this control and showed a monophasic increase in the GSK-3β level in response to insulin. Administration of GSK-3β inhibitors lithium chloride and L803-mts restored glucose homeostasis and rescued the glucose intolerance and impaired insulin response in L-Stat3 -/- mice. CONCLUSIONS-These data indicate that STAT3 sensitizes insulin signaling by negatively regulating GSK-3β. Inactivation of STAT3 in the liver contributes significantly to the pathogenesis of insulin resistance.

AB - OBJECTIVE-Glucose homeostasis is achieved by triggering regulation of glycogen synthesis genes in response to insulin when mammals feed, but the underlying molecular mechanism remains largely unknown. The aim of our study was to examine the role of the signal transducers and activators of transcription 3 (STAT3) in insulin signaling. RESEARCH DESIGN AND METHODS-We generated a strain of mice carrying a targeted disruption of Stat3 gene in the liver (L-Stat3 -/- mice). Hepatocytes of the L-Stat3 -/- mice were isolated to establish cell lines for mechanistic studies. Nuclear translocation and DNA-protein interaction of STAT3 was analyzed with immunofluorescent and chromatin immunopre-cipitation methods, respectively. Levels of glucose, insulin, lep-tin, and glucagon were profiled, and putative downstream molecules of STAT3 were examined in the presence of various stimuli in L-Stat3 -/- and control mice. RESULTS-STAT3 was found to sensitize the insulin signaling through suppression of GSK-3β, a negative regulator of insulin signaling pathway. During feeding, both mRNA and protein levels of GSK-3β decreased in Stat3 f/+ mice, which reflected the need of hepatocytes for insulin to induce glycogen synthesis. In contrast, the L-Stat3 -/- mice lost this control and showed a monophasic increase in the GSK-3β level in response to insulin. Administration of GSK-3β inhibitors lithium chloride and L803-mts restored glucose homeostasis and rescued the glucose intolerance and impaired insulin response in L-Stat3 -/- mice. CONCLUSIONS-These data indicate that STAT3 sensitizes insulin signaling by negatively regulating GSK-3β. Inactivation of STAT3 in the liver contributes significantly to the pathogenesis of insulin resistance.

UR - http://www.scopus.com/inward/record.url?scp=48449095742&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=48449095742&partnerID=8YFLogxK

U2 - 10.2337/db06-1582

DO - 10.2337/db06-1582

M3 - Article

VL - 57

SP - 1227

EP - 1235

JO - Diabetes

JF - Diabetes

SN - 0012-1797

IS - 5

ER -