Static and dynamic components of renal cortical brush border and basolateral membrane fluidity

Role of cholesterol

Bruce Molitoris, Catherine Hoilien

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

Static polarization and differential polarized phase fluorimetry studies on rat renal cortical brush border (BBM) and basolateral membranes (BLM) were undertaken to determine the membrane components responsible for differences in BBM and BLM fluidity, whether these differences were due to the order or dynamic components of membrane fluidity and if a fluidity gradient existed within the bilayer. Surface membrane proteins rigidified both BBM and BLM fluidity. Neutral lipid extraction, on the other hand, caused a larger decrease in BBM than BLM fluorescence polarization (0.104 vs. 0.60, Pα) (0.212 vs. 0.154, P

Original languageEnglish (US)
Pages (from-to)165-172
Number of pages8
JournalJournal of Membrane Biology
Volume99
Issue number3
DOIs
StatePublished - Nov 1987
Externally publishedYes

Fingerprint

Membrane Fluidity
Microvilli
Cholesterol
Kidney
Membranes
Membrane Proteins
Fluorometry
Fluorescence Polarization
Lipids

Keywords

  • basolateral membranes
  • brush border membranes
  • cholesterol
  • fluorescence polarization
  • renal

ASJC Scopus subject areas

  • Physiology
  • Cell Biology
  • Biophysics

Cite this

Static and dynamic components of renal cortical brush border and basolateral membrane fluidity : Role of cholesterol. / Molitoris, Bruce; Hoilien, Catherine.

In: Journal of Membrane Biology, Vol. 99, No. 3, 11.1987, p. 165-172.

Research output: Contribution to journalArticle

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