Statistical analysis of membrane proteome expression changes in Saccharomyces cerevisiae

Boris Zybailov, Amber Mosley, Mihaela E. Sardiu, Michael K. Coleman, Laurence Florens, Michael P. Washburn

Research output: Contribution to journalArticle

657 Citations (Scopus)

Abstract

We have devised an approach for analyzing shotgun proteomics datasets based on the normalized spectral abundance factor that can be used for quantitative proteomics analysis. Three biological replicates of samples enriched for plasma membranes were isolated from S. cerevisiae grown in 14N-rich media and 15N-minimal media and analyzed via quantitative multidimensional protein identification technology. The natural log transformation of NSAF values from S. cerevisiae cells grown in 14N YPD media and 15N-minimal media had a normal distribution. The t-test analysis demonstrated 221 of 1316 proteins were significantly overexpressed in one or the other growth conditions with a p value

Original languageEnglish (US)
Pages (from-to)2339-2347
Number of pages9
JournalJournal of Proteome Research
Volume5
Issue number9
DOIs
StatePublished - Sep 2006
Externally publishedYes

Fingerprint

Proteome
Proteomics
Yeast
Saccharomyces cerevisiae
Statistical methods
Membranes
Normal Distribution
Firearms
Normal distribution
Cell membranes
Proteins
Cells
Cell Membrane
Technology
Growth
Datasets

Keywords

  • Integral membrane proteins
  • Mass spectrometry
  • Multidimensional protein identification technology
  • Quantitative proteomics
  • Statistical analysis

ASJC Scopus subject areas

  • Genetics
  • Biotechnology
  • Biochemistry

Cite this

Statistical analysis of membrane proteome expression changes in Saccharomyces cerevisiae. / Zybailov, Boris; Mosley, Amber; Sardiu, Mihaela E.; Coleman, Michael K.; Florens, Laurence; Washburn, Michael P.

In: Journal of Proteome Research, Vol. 5, No. 9, 09.2006, p. 2339-2347.

Research output: Contribution to journalArticle

Zybailov, Boris ; Mosley, Amber ; Sardiu, Mihaela E. ; Coleman, Michael K. ; Florens, Laurence ; Washburn, Michael P. / Statistical analysis of membrane proteome expression changes in Saccharomyces cerevisiae. In: Journal of Proteome Research. 2006 ; Vol. 5, No. 9. pp. 2339-2347.
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AU - Washburn, Michael P.

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