Staurosporine enhanced benzamide riboside-induced apoptosis in human multidrug-resistant promyelocytic leukemia cells (HL-60/VCR) in vitro

L. Hunáková, J. Duraj, D. Romanová, L. Novotný, J. Sedlák, M. R. Kelley, T. Szekeres, H. N. Jayaram, B. Chokváth

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8 Scopus citations

Abstract

The inosine monophosphate (IMP) dehydrogenase inhibitor benzamide riboside (BR) induced apoptosis (detected with the aid of flow cytometric identification of cells with sub-G0 DNA content and increased side and angle light scatter) equally or slightly more intensively in the multidrug- resistant human promyelocytic leukemia cell line (HL-60/VCR: MDR-1 gene, Pgp positive in comparison with the parental drug sensitive HL-60 cells. Staurosporine alone induced relatively low level of apoptosis in parental HL- 60 cells but higher level (approximately 35%) of apoptosis in multidrug- resistant HL-60/VCR cells after 24 hour induction. The combination of benzamide riboside and staurosporine induced in both drug-sensitive and drug- resistant HL-60 cells a marked proportion of apoptotic cells already after short (6 hour) induction (more than 30% of apoptotic cells).

Original languageEnglish (US)
Pages (from-to)204-209
Number of pages6
JournalNeoplasma
Volume45
Issue number4
StatePublished - Jan 1 1998

Keywords

  • Apoptosis
  • Benzamide riboside
  • Drug-resistant
  • Flow cytometry
  • Human leukemia cells
  • MDR-1
  • P-glycoprotein
  • Staurosporine

ASJC Scopus subject areas

  • Cancer Research

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  • Cite this

    Hunáková, L., Duraj, J., Romanová, D., Novotný, L., Sedlák, J., Kelley, M. R., Szekeres, T., Jayaram, H. N., & Chokváth, B. (1998). Staurosporine enhanced benzamide riboside-induced apoptosis in human multidrug-resistant promyelocytic leukemia cells (HL-60/VCR) in vitro. Neoplasma, 45(4), 204-209.