Staying straight with A-tracts: A DNA analog of the HIV-1 polypurine tract

Marie L. Coté, Mark Pflomm, Millie Georgiadis

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

The polypurine tract (PPT) from the HIV-1 genome is resistant to digestion by reverse transcriptase following (-)-strand synthesis and is used to prime (+)-strand synthesis during retroviral replication. We have determined the crystal structure of the asymmetric DNA/DNA analog16-mer duplex (CTTTTTAAAAGAAAAG/CTTTTCTTTTAAAAAG) comprising most of the "visible" portion of the RNA:DNA hybrid from the polypurine tract of HIV-1, which was previously reported in a complex with HIV-1 reverse transcriptase. Our 16-mer completely encompasses a 10-mer DNA duplex analog of the HIV-1 PPT. We report here a detailed analysis of our B′ form 16-mer DNA structure, including three full pure A-tracts, as well as a comparative structural analysis with polypurine tract and other A-tract-containing nucleic acid structures. Our analysis reveals that the polypurine tract structures share structural features despite being different nucleic acid forms (i.e. DNA:DNA versus RNA:DNA). In addition, the previously reported A-tract-containing DNA molecules bound to topoisomerase I are remarkably similar to our polypurine tract 16-mer structure. On the basis of our analysis, we suggest that the specific topology of long pure A-tracts is remarkably comparable across a wide array of biological environments.

Original languageEnglish
Pages (from-to)57-74
Number of pages18
JournalJournal of Molecular Biology
Volume330
Issue number1
DOIs
StatePublished - Jun 27 2003

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HIV-1
DNA
Nucleic Acids
RNA
Type I DNA Topoisomerase
RNA-Directed DNA Polymerase
Digestion
Genome

Keywords

  • A-tract
  • HIV-1 polypurine tract
  • Moloney murine leukemia virus
  • Nucleic acid
  • Protein-DNA complex

ASJC Scopus subject areas

  • Virology

Cite this

Staying straight with A-tracts : A DNA analog of the HIV-1 polypurine tract. / Coté, Marie L.; Pflomm, Mark; Georgiadis, Millie.

In: Journal of Molecular Biology, Vol. 330, No. 1, 27.06.2003, p. 57-74.

Research output: Contribution to journalArticle

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