Stimulation of DNA synthesis by PDGF in the newt cardiac myocyte

M. H. Soonpaa, J. O. Oberpriller, J. C. Oberpriller

Research output: Contribution to journalArticle

17 Scopus citations

Abstract

The adult newt cardiac ventricular myocyte has been successfully placed in cell culture and has been shown to undergo in vitro DNA synthess. Although several growth factors have been reported to increase DNA synthesis in cardiac myocytes in vitro, PDGF has not been reported to do so, but has been shown to be active in other systems. Ventricles were removed from the adult red-spotted newt and were enzymatically and mechanically dissociated in a solution containing trypsin and collagenase. Cells were preplated on to plastic to remove non-myocytes. Myocytes were then plated onto laminin. Groups of myocytes were fed control medium and medium containing porcine PDGF. Myocytes were given 1 μCi/ml of tritiated thymidine 6 or 24 h before fixation. Control myocytes showed a peak DNA synthesis at 12-14 days in culture. One ng/ml of PDGF increased DNA synthesis significantly to 22% above control. Myocytes responded to PDGF with significantly increased DNA synthesis in about 12 h. PDGF did not induce earlier DNA synthesis, but increased synthesis at all days of culture tested. These results indicate that PDGF acts upon cardiac myocytes, increasing their DNA synthesis.

Original languageEnglish (US)
Pages (from-to)1039-1046
Number of pages8
JournalJournal of Molecular and Cellular Cardiology
Volume24
Issue number9
DOIs
StatePublished - Sep 1992

    Fingerprint

Keywords

  • Cardiac myocyte
  • Cell division
  • DNA synthesis
  • PDGF

ASJC Scopus subject areas

  • Molecular Biology
  • Cardiology and Cardiovascular Medicine

Cite this