Stimulation of erythroid colony formation in vitro by erythropoietin immobilized on agarose-bound lectins

G. David Roodman, J. L. Spivak, E. D. Zanjani

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

WGA and the PHA-E covalently linked to beaded agarose were employed to prepare an immobilized form of Ep. Agarose-PHA-E-Ep complexes induced the formation of sheep and human erythroid colonies in plasma clot and methylcellulose cultures without detectable leaching of Ep. Approximately 85% to 95% of the colonies formed were attached or juxtaposed to the agarose beads. The dose-response curve of the agarose-PHA-E-Ep complexes for erythroid colony formation was similar to that of unbound Ep, and after removal of cells adherent to the beads, the immobilized Ep could be reused. Attachment of erythroid colonies to agarose-lectin-Ep complexes was due to lectin-cell surface interactions. These results support the concept that Ep acts at the surface of its target cells.

Original languageEnglish (US)
Pages (from-to)684-690
Number of pages7
JournalJournal of Laboratory and Clinical Medicine
Volume98
Issue number5
StatePublished - 1981
Externally publishedYes

Fingerprint

Erythropoietin
Lectins
Sepharose
Methylcellulose
Cell Communication
Leaching
Sheep
In Vitro Techniques
Plasmas
erythroagglutinating phytohemagglutinin

ASJC Scopus subject areas

  • Medicine(all)
  • Pathology and Forensic Medicine

Cite this

Stimulation of erythroid colony formation in vitro by erythropoietin immobilized on agarose-bound lectins. / Roodman, G. David; Spivak, J. L.; Zanjani, E. D.

In: Journal of Laboratory and Clinical Medicine, Vol. 98, No. 5, 1981, p. 684-690.

Research output: Contribution to journalArticle

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