Stimulation of human and rat islet β-cell proliferation with retention of function by the homeodomain transcription factor Nkx6.1

Jonathan C. Schisler, Patrick T. Fueger, Daniella A. Babu, Hans E. Hohmeier, Jeffery S. Tessem, Danhong Lu, Thomas C. Becker, Bashoo Naziruddin, Marlon Levy, Raghu Mirmira, Christopher B. Newgard

Research output: Contribution to journalArticle

71 Citations (Scopus)

Abstract

The homeodomain transcription factor Nkx6.1 plays an important role in pancreatic islet β-cell development, but its effects on adult β-cell function, survival, and proliferation are not well understood. In the present study, we demonstrated that treatment of primary rat pancreatic islets with a cytomegalovirus promoter-driven recombinant adenovirus containing the Nkx6.1 cDNA (AdCMV-Nkx6.1) causes dramatic increases in [methyl-3H] thymidine and 5-bromo-2′-deoxyuridine (BrdU) incorporation and in the number of cells per islet relative to islets treated with a control adenovirus (AdCMV-βGAL), whereas suppression of Nkx6.1 expression reduces thymidine incorporation. Immunocytochemical studies reveal that >80% of BrdU-positive cells in AdCMV-Nkx6.1-treated islets are β cells. Microarray, real-time PCR, and immunoblot analyses reveal that overexpression of Nkx6.1 in rat islets causes concerted upregulation of a cadre of cell cycle control genes, including those encoding cyclins A, B, and E, and several regulatory kinases. Cyclin E is upregulated earlier than the other cyclins, and adenovirus-mediated overexpression of cyclin E is shown to be sufficient to activate islet cell proliferation. Moreover, chromatin immunoprecipitation assays demonstrate direct interaction of Nkx6.1 with the cyclin A2 and B1 genes. Overexpression of Nkx6.1 in rat islets caused a clear enhancement of glucose-stimulated insulin secretion (GSIS), whereas overexpression of Nkx6.1 in human islets caused an increase in the level of [3H] thymidine incorporation that was twice the control level, along with complete retention of GSIS. We conclude that Nkx6.1 is among the very rare factors capable of stimulating β-cell replication with retention or enhancement of function, properties that may be exploitable for expansion of β-cell mass in treatment of both major forms of diabetes.

Original languageEnglish (US)
Pages (from-to)3465-3476
Number of pages12
JournalMolecular and Cellular Biology
Volume28
Issue number10
DOIs
StatePublished - May 2008
Externally publishedYes

Fingerprint

Islets of Langerhans
Transcription Factors
Cell Proliferation
Cyclin E
Adenoviridae
Thymidine
Bromodeoxyuridine
Cyclin A2
Insulin
Cyclin B
Cyclin B1
Glucose
Cyclin A
cdc Genes
Cyclins
Chromatin Immunoprecipitation
Cell Cycle Checkpoints
Cytomegalovirus
Real-Time Polymerase Chain Reaction
Cell Survival

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

Schisler, J. C., Fueger, P. T., Babu, D. A., Hohmeier, H. E., Tessem, J. S., Lu, D., ... Newgard, C. B. (2008). Stimulation of human and rat islet β-cell proliferation with retention of function by the homeodomain transcription factor Nkx6.1. Molecular and Cellular Biology, 28(10), 3465-3476. https://doi.org/10.1128/MCB.01791-07

Stimulation of human and rat islet β-cell proliferation with retention of function by the homeodomain transcription factor Nkx6.1. / Schisler, Jonathan C.; Fueger, Patrick T.; Babu, Daniella A.; Hohmeier, Hans E.; Tessem, Jeffery S.; Lu, Danhong; Becker, Thomas C.; Naziruddin, Bashoo; Levy, Marlon; Mirmira, Raghu; Newgard, Christopher B.

In: Molecular and Cellular Biology, Vol. 28, No. 10, 05.2008, p. 3465-3476.

Research output: Contribution to journalArticle

Schisler, JC, Fueger, PT, Babu, DA, Hohmeier, HE, Tessem, JS, Lu, D, Becker, TC, Naziruddin, B, Levy, M, Mirmira, R & Newgard, CB 2008, 'Stimulation of human and rat islet β-cell proliferation with retention of function by the homeodomain transcription factor Nkx6.1', Molecular and Cellular Biology, vol. 28, no. 10, pp. 3465-3476. https://doi.org/10.1128/MCB.01791-07
Schisler, Jonathan C. ; Fueger, Patrick T. ; Babu, Daniella A. ; Hohmeier, Hans E. ; Tessem, Jeffery S. ; Lu, Danhong ; Becker, Thomas C. ; Naziruddin, Bashoo ; Levy, Marlon ; Mirmira, Raghu ; Newgard, Christopher B. / Stimulation of human and rat islet β-cell proliferation with retention of function by the homeodomain transcription factor Nkx6.1. In: Molecular and Cellular Biology. 2008 ; Vol. 28, No. 10. pp. 3465-3476.
@article{3b69a55bddb142a693e980dff3941939,
title = "Stimulation of human and rat islet β-cell proliferation with retention of function by the homeodomain transcription factor Nkx6.1",
abstract = "The homeodomain transcription factor Nkx6.1 plays an important role in pancreatic islet β-cell development, but its effects on adult β-cell function, survival, and proliferation are not well understood. In the present study, we demonstrated that treatment of primary rat pancreatic islets with a cytomegalovirus promoter-driven recombinant adenovirus containing the Nkx6.1 cDNA (AdCMV-Nkx6.1) causes dramatic increases in [methyl-3H] thymidine and 5-bromo-2′-deoxyuridine (BrdU) incorporation and in the number of cells per islet relative to islets treated with a control adenovirus (AdCMV-βGAL), whereas suppression of Nkx6.1 expression reduces thymidine incorporation. Immunocytochemical studies reveal that >80{\%} of BrdU-positive cells in AdCMV-Nkx6.1-treated islets are β cells. Microarray, real-time PCR, and immunoblot analyses reveal that overexpression of Nkx6.1 in rat islets causes concerted upregulation of a cadre of cell cycle control genes, including those encoding cyclins A, B, and E, and several regulatory kinases. Cyclin E is upregulated earlier than the other cyclins, and adenovirus-mediated overexpression of cyclin E is shown to be sufficient to activate islet cell proliferation. Moreover, chromatin immunoprecipitation assays demonstrate direct interaction of Nkx6.1 with the cyclin A2 and B1 genes. Overexpression of Nkx6.1 in rat islets caused a clear enhancement of glucose-stimulated insulin secretion (GSIS), whereas overexpression of Nkx6.1 in human islets caused an increase in the level of [3H] thymidine incorporation that was twice the control level, along with complete retention of GSIS. We conclude that Nkx6.1 is among the very rare factors capable of stimulating β-cell replication with retention or enhancement of function, properties that may be exploitable for expansion of β-cell mass in treatment of both major forms of diabetes.",
author = "Schisler, {Jonathan C.} and Fueger, {Patrick T.} and Babu, {Daniella A.} and Hohmeier, {Hans E.} and Tessem, {Jeffery S.} and Danhong Lu and Becker, {Thomas C.} and Bashoo Naziruddin and Marlon Levy and Raghu Mirmira and Newgard, {Christopher B.}",
year = "2008",
month = "5",
doi = "10.1128/MCB.01791-07",
language = "English (US)",
volume = "28",
pages = "3465--3476",
journal = "Molecular and Cellular Biology",
issn = "0270-7306",
publisher = "American Society for Microbiology",
number = "10",

}

TY - JOUR

T1 - Stimulation of human and rat islet β-cell proliferation with retention of function by the homeodomain transcription factor Nkx6.1

AU - Schisler, Jonathan C.

AU - Fueger, Patrick T.

AU - Babu, Daniella A.

AU - Hohmeier, Hans E.

AU - Tessem, Jeffery S.

AU - Lu, Danhong

AU - Becker, Thomas C.

AU - Naziruddin, Bashoo

AU - Levy, Marlon

AU - Mirmira, Raghu

AU - Newgard, Christopher B.

PY - 2008/5

Y1 - 2008/5

N2 - The homeodomain transcription factor Nkx6.1 plays an important role in pancreatic islet β-cell development, but its effects on adult β-cell function, survival, and proliferation are not well understood. In the present study, we demonstrated that treatment of primary rat pancreatic islets with a cytomegalovirus promoter-driven recombinant adenovirus containing the Nkx6.1 cDNA (AdCMV-Nkx6.1) causes dramatic increases in [methyl-3H] thymidine and 5-bromo-2′-deoxyuridine (BrdU) incorporation and in the number of cells per islet relative to islets treated with a control adenovirus (AdCMV-βGAL), whereas suppression of Nkx6.1 expression reduces thymidine incorporation. Immunocytochemical studies reveal that >80% of BrdU-positive cells in AdCMV-Nkx6.1-treated islets are β cells. Microarray, real-time PCR, and immunoblot analyses reveal that overexpression of Nkx6.1 in rat islets causes concerted upregulation of a cadre of cell cycle control genes, including those encoding cyclins A, B, and E, and several regulatory kinases. Cyclin E is upregulated earlier than the other cyclins, and adenovirus-mediated overexpression of cyclin E is shown to be sufficient to activate islet cell proliferation. Moreover, chromatin immunoprecipitation assays demonstrate direct interaction of Nkx6.1 with the cyclin A2 and B1 genes. Overexpression of Nkx6.1 in rat islets caused a clear enhancement of glucose-stimulated insulin secretion (GSIS), whereas overexpression of Nkx6.1 in human islets caused an increase in the level of [3H] thymidine incorporation that was twice the control level, along with complete retention of GSIS. We conclude that Nkx6.1 is among the very rare factors capable of stimulating β-cell replication with retention or enhancement of function, properties that may be exploitable for expansion of β-cell mass in treatment of both major forms of diabetes.

AB - The homeodomain transcription factor Nkx6.1 plays an important role in pancreatic islet β-cell development, but its effects on adult β-cell function, survival, and proliferation are not well understood. In the present study, we demonstrated that treatment of primary rat pancreatic islets with a cytomegalovirus promoter-driven recombinant adenovirus containing the Nkx6.1 cDNA (AdCMV-Nkx6.1) causes dramatic increases in [methyl-3H] thymidine and 5-bromo-2′-deoxyuridine (BrdU) incorporation and in the number of cells per islet relative to islets treated with a control adenovirus (AdCMV-βGAL), whereas suppression of Nkx6.1 expression reduces thymidine incorporation. Immunocytochemical studies reveal that >80% of BrdU-positive cells in AdCMV-Nkx6.1-treated islets are β cells. Microarray, real-time PCR, and immunoblot analyses reveal that overexpression of Nkx6.1 in rat islets causes concerted upregulation of a cadre of cell cycle control genes, including those encoding cyclins A, B, and E, and several regulatory kinases. Cyclin E is upregulated earlier than the other cyclins, and adenovirus-mediated overexpression of cyclin E is shown to be sufficient to activate islet cell proliferation. Moreover, chromatin immunoprecipitation assays demonstrate direct interaction of Nkx6.1 with the cyclin A2 and B1 genes. Overexpression of Nkx6.1 in rat islets caused a clear enhancement of glucose-stimulated insulin secretion (GSIS), whereas overexpression of Nkx6.1 in human islets caused an increase in the level of [3H] thymidine incorporation that was twice the control level, along with complete retention of GSIS. We conclude that Nkx6.1 is among the very rare factors capable of stimulating β-cell replication with retention or enhancement of function, properties that may be exploitable for expansion of β-cell mass in treatment of both major forms of diabetes.

UR - http://www.scopus.com/inward/record.url?scp=43249122991&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=43249122991&partnerID=8YFLogxK

U2 - 10.1128/MCB.01791-07

DO - 10.1128/MCB.01791-07

M3 - Article

C2 - 18347054

AN - SCOPUS:43249122991

VL - 28

SP - 3465

EP - 3476

JO - Molecular and Cellular Biology

JF - Molecular and Cellular Biology

SN - 0270-7306

IS - 10

ER -