Stimulation of interleukin‐6 production by either calcitonin gene‐related peptide or parathyroid hormone in two phenotypically distinct bone marrow‐derived murine stromal cell lines

Y. Sakagami, G. Girasole, X. ‐P Yu, H. S. Boswell, Stavros C. Manolagas

Research output: Contribution to journalArticle

43 Scopus citations

Abstract

It was recently shown that interleukin-6 (IL-6) is produced by bone and bone marrow-derived stromal cells and that it plays an important role in osteoclast development. Here we examined whether parathyroid hormone (PTH), calcitonin (CT), or the calcitonin gene-related peptide (CGRP) influence IL-6 production by two murine bone marrow-derived stromal cell lines: the preadipocyte-like stromal cell line +/+ LDA11 and the fibroendothelial stromal cell line MBA 13.2. We found that CGRP (but not PTH or CT) exerted a dose-dependent increase in cAMP and IL-6 production in the +/+ LDAII cells. In addition, CGRP had an inhibiting effect on the proliferation of this stromal cell line. CGRP, however, did not affect cAMP or IL-6 in the rat osteogenic sarcoma cell line UMR-106-06, which exhibits CT receptors, whereas CT stimulated both cAMP and IL-6 by the UMR-106-01 cells. In contrast to the specificity of the IL-6 response of the +/+ LDA11 cells to CGRP, IL-6 production by the MBA 13.2 stromal cells was stimulated by PTH whereas CGRP or CT had no effect. These data suggest that bone marrow-derived stromal cells express receptors for either CGRP or PTH in a phenotype-specific manner and that, acting via these receptors, CGRP and PTH stimulate IL-6 production by stromal cells. In addition, the evidence for specific receptors for the neuropeptide CGRP in bone marrow stromal cells and an effect of CGRP on IL-6 raises the possibility for a role of cytokines in a putative interplay between neuronal stimuli and bone.

Original languageEnglish (US)
Pages (from-to)811-816
Number of pages6
JournalJournal of Bone and Mineral Research
Volume8
Issue number7
DOIs
StatePublished - Jul 1993

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Orthopedics and Sports Medicine

Fingerprint Dive into the research topics of 'Stimulation of interleukin‐6 production by either calcitonin gene‐related peptide or parathyroid hormone in two phenotypically distinct bone marrow‐derived murine stromal cell lines'. Together they form a unique fingerprint.

  • Cite this