Stimulation of mouse and human primitive hematopoiesis by murine embryonic aorta-gonad-mesonephros-derived stromal cell lines

Ming Jiang Xu, Kohichiro Tsuji, Takahiro Ueda, Yoh Suke Mukouyama, Takahiko Hara, Feng Chun Yang, Yasuhiro Ebihara, Sahoko Matsuoka, Atsushi Manabe, Akira Kikuchi, Mamoru Ito, Atsushi Miyajima, Tatsutoshi Nakahata

Research output: Contribution to journalArticle

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Abstract

We report here on a novel stromal cell line, AGM-S3 derived from the aorta-gonad-mesonephros (AGM) region of a 10.5 days postcoitum (dpc) mouse embryo. The AGM-S3 cells promoted production of hematopoietic progenitors and day-12 spleen colony-forming cells from Lin-c-Kit+Sca-1+ murine primitive hematopoietic cells. They also supported for 6 weeks generation of human multipotential progenitors from cord blood CD34+CD38- primitive hematopoietic cells. Human long-term repopulating hematopoietic stem cells (LTR-HSC) with the potential to reconstitute hematopoiesis in NOD/SCID mice were maintained on AGM-S3 cells for at least 4 weeks. Flow cytometric analysis showed that CD13, vascular cellular adhesion molecule-1, and Sca-1 were expressed on AGM-S3 cells. Because stem cell factor, interleukin-6 (IL- 6), and oncostatin M, but not IL-3, IL-11, leukemia-inhibitory factor, granulocyte colony-stimulating factor, granulocyte-macrophage colony- stimulating factor, thrombopoietin, and Flk2 ligand were detected in reverse transcription-polymerase chain reaction analysis of AGM-S3 cells, the cells seem to express species-cross reactive molecule(s) other than the cytokines examined and which act on primitive hematopoietic progenitor/stem cells. This cell line is expected to elucidate molecular mechanisms regulating early hematopoiesis and pave the way for developing strategies for expansion of human transplantable HSC.

Original languageEnglish (US)
Pages (from-to)2032-2040
Number of pages9
JournalBlood
Volume92
Issue number6
StatePublished - Sep 15 1998
Externally publishedYes

Fingerprint

Mesonephros
Hematopoiesis
Gonads
Stromal Cells
Stem cells
Aorta
Cells
Oncostatin M
Interleukin-11
Thrombopoietin
Leukemia Inhibitory Factor
Cell Line
Molecules
Stem Cell Factor
Interleukin-3
Polymerase chain reaction
Granulocyte Colony-Stimulating Factor
Transcription
Granulocyte-Macrophage Colony-Stimulating Factor
Interleukin-6

ASJC Scopus subject areas

  • Hematology

Cite this

Xu, M. J., Tsuji, K., Ueda, T., Mukouyama, Y. S., Hara, T., Yang, F. C., ... Nakahata, T. (1998). Stimulation of mouse and human primitive hematopoiesis by murine embryonic aorta-gonad-mesonephros-derived stromal cell lines. Blood, 92(6), 2032-2040.

Stimulation of mouse and human primitive hematopoiesis by murine embryonic aorta-gonad-mesonephros-derived stromal cell lines. / Xu, Ming Jiang; Tsuji, Kohichiro; Ueda, Takahiro; Mukouyama, Yoh Suke; Hara, Takahiko; Yang, Feng Chun; Ebihara, Yasuhiro; Matsuoka, Sahoko; Manabe, Atsushi; Kikuchi, Akira; Ito, Mamoru; Miyajima, Atsushi; Nakahata, Tatsutoshi.

In: Blood, Vol. 92, No. 6, 15.09.1998, p. 2032-2040.

Research output: Contribution to journalArticle

Xu, MJ, Tsuji, K, Ueda, T, Mukouyama, YS, Hara, T, Yang, FC, Ebihara, Y, Matsuoka, S, Manabe, A, Kikuchi, A, Ito, M, Miyajima, A & Nakahata, T 1998, 'Stimulation of mouse and human primitive hematopoiesis by murine embryonic aorta-gonad-mesonephros-derived stromal cell lines', Blood, vol. 92, no. 6, pp. 2032-2040.
Xu MJ, Tsuji K, Ueda T, Mukouyama YS, Hara T, Yang FC et al. Stimulation of mouse and human primitive hematopoiesis by murine embryonic aorta-gonad-mesonephros-derived stromal cell lines. Blood. 1998 Sep 15;92(6):2032-2040.
Xu, Ming Jiang ; Tsuji, Kohichiro ; Ueda, Takahiro ; Mukouyama, Yoh Suke ; Hara, Takahiko ; Yang, Feng Chun ; Ebihara, Yasuhiro ; Matsuoka, Sahoko ; Manabe, Atsushi ; Kikuchi, Akira ; Ito, Mamoru ; Miyajima, Atsushi ; Nakahata, Tatsutoshi. / Stimulation of mouse and human primitive hematopoiesis by murine embryonic aorta-gonad-mesonephros-derived stromal cell lines. In: Blood. 1998 ; Vol. 92, No. 6. pp. 2032-2040.
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AU - Tsuji, Kohichiro

AU - Ueda, Takahiro

AU - Mukouyama, Yoh Suke

AU - Hara, Takahiko

AU - Yang, Feng Chun

AU - Ebihara, Yasuhiro

AU - Matsuoka, Sahoko

AU - Manabe, Atsushi

AU - Kikuchi, Akira

AU - Ito, Mamoru

AU - Miyajima, Atsushi

AU - Nakahata, Tatsutoshi

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N2 - We report here on a novel stromal cell line, AGM-S3 derived from the aorta-gonad-mesonephros (AGM) region of a 10.5 days postcoitum (dpc) mouse embryo. The AGM-S3 cells promoted production of hematopoietic progenitors and day-12 spleen colony-forming cells from Lin-c-Kit+Sca-1+ murine primitive hematopoietic cells. They also supported for 6 weeks generation of human multipotential progenitors from cord blood CD34+CD38- primitive hematopoietic cells. Human long-term repopulating hematopoietic stem cells (LTR-HSC) with the potential to reconstitute hematopoiesis in NOD/SCID mice were maintained on AGM-S3 cells for at least 4 weeks. Flow cytometric analysis showed that CD13, vascular cellular adhesion molecule-1, and Sca-1 were expressed on AGM-S3 cells. Because stem cell factor, interleukin-6 (IL- 6), and oncostatin M, but not IL-3, IL-11, leukemia-inhibitory factor, granulocyte colony-stimulating factor, granulocyte-macrophage colony- stimulating factor, thrombopoietin, and Flk2 ligand were detected in reverse transcription-polymerase chain reaction analysis of AGM-S3 cells, the cells seem to express species-cross reactive molecule(s) other than the cytokines examined and which act on primitive hematopoietic progenitor/stem cells. This cell line is expected to elucidate molecular mechanisms regulating early hematopoiesis and pave the way for developing strategies for expansion of human transplantable HSC.

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