Stranded whole transcriptome RNA-Seq for all RNA types

David F B Miller, Pearlly X. Yan, Fang Fang, Aaron Buechlein, James B. Ford, Haixu Tang, Tim H. Huang, Matthew E. Burow, Yunlong Liu, Douglas B. Rusch, Kenneth Nephew

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Stranded whole transcriptome RNA-Seq described in this unit captures quantitative expression data for all types of RNA including, but not limited to, miRNA (microRNA), piRNA(Piwi-interacting RNA), snoRNA(small nucleolar RNA), lincRNA(large non-coding intergenicRNA), SRPRNA(signal recognition particle RNA), tRNA (transfer RNA), mtRNA (mitochondrial RNA), and mRNA (messenger RNA). The size and nature of these types of RNA are irrelevant to the approach described here. Barcoded libraries for multiplexing on the Illumina platform are generated with this approach but it can be applied to other platforms with a few modifications.

Original languageEnglish (US)
Pages (from-to)11.14.1-11.14.23
JournalCurrent protocols in human genetics / editorial board, Jonathan L. Haines ... [et al.]
Volume2015
DOIs
StatePublished - 2015

Fingerprint

Transcriptome
RNA
Signal Recognition Particle
Small Nucleolar RNA
Transfer RNA
MicroRNAs
Small Interfering RNA
Libraries
Messenger RNA

Keywords

  • Duplex-specific nuclease
  • Gene expression
  • RNA-Seq
  • Transcriptome

ASJC Scopus subject areas

  • Genetics
  • Genetics(clinical)

Cite this

Stranded whole transcriptome RNA-Seq for all RNA types. / Miller, David F B; Yan, Pearlly X.; Fang, Fang; Buechlein, Aaron; Ford, James B.; Tang, Haixu; Huang, Tim H.; Burow, Matthew E.; Liu, Yunlong; Rusch, Douglas B.; Nephew, Kenneth.

In: Current protocols in human genetics / editorial board, Jonathan L. Haines ... [et al.], Vol. 2015, 2015, p. 11.14.1-11.14.23.

Research output: Contribution to journalArticle

Miller, DFB, Yan, PX, Fang, F, Buechlein, A, Ford, JB, Tang, H, Huang, TH, Burow, ME, Liu, Y, Rusch, DB & Nephew, K 2015, 'Stranded whole transcriptome RNA-Seq for all RNA types', Current protocols in human genetics / editorial board, Jonathan L. Haines ... [et al.], vol. 2015, pp. 11.14.1-11.14.23. https://doi.org/10.1002/0471142905.hg1114s84
Miller, David F B ; Yan, Pearlly X. ; Fang, Fang ; Buechlein, Aaron ; Ford, James B. ; Tang, Haixu ; Huang, Tim H. ; Burow, Matthew E. ; Liu, Yunlong ; Rusch, Douglas B. ; Nephew, Kenneth. / Stranded whole transcriptome RNA-Seq for all RNA types. In: Current protocols in human genetics / editorial board, Jonathan L. Haines ... [et al.]. 2015 ; Vol. 2015. pp. 11.14.1-11.14.23.
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