Stranded whole transcriptome RNA-Seq for all RNA types

David F.B. Miller, Pearlly X. Yan, Fang Fang, Aaron Buechlein, James B. Ford, Haixu Tang, Tim H. Huang, Matthew E. Burow, Yunlong Liu, Douglas B. Rusch, Kenneth P. Nephew

Research output: Contribution to journalArticle

5 Scopus citations

Abstract

Stranded whole transcriptome RNA-Seq described in this unit captures quantitative expression data for all types of RNA including, but not limited to, miRNA (microRNA), piRNA(Piwi-interacting RNA), snoRNA(small nucleolar RNA), lincRNA(large non-coding intergenicRNA), SRPRNA(signal recognition particle RNA), tRNA (transfer RNA), mtRNA (mitochondrial RNA), and mRNA (messenger RNA). The size and nature of these types of RNA are irrelevant to the approach described here. Barcoded libraries for multiplexing on the Illumina platform are generated with this approach but it can be applied to other platforms with a few modifications.

Original languageEnglish (US)
Pages (from-to)11.14.1-11.14.23
JournalCurrent Protocols in Human Genetics
Volume2015
DOIs
StatePublished - Jan 1 2015

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Keywords

  • Duplex-specific nuclease
  • Gene expression
  • RNA-Seq
  • Transcriptome

ASJC Scopus subject areas

  • Genetics
  • Genetics(clinical)

Cite this

Miller, D. F. B., Yan, P. X., Fang, F., Buechlein, A., Ford, J. B., Tang, H., Huang, T. H., Burow, M. E., Liu, Y., Rusch, D. B., & Nephew, K. P. (2015). Stranded whole transcriptome RNA-Seq for all RNA types. Current Protocols in Human Genetics, 2015, 11.14.1-11.14.23. https://doi.org/10.1002/0471142905.hg1114s84