Streptozotocin-Induced Diabetes Increases γ-Glutamyltranspeptidase Activity but not Expression in Rat Liver

John B. Watkins, James E. Klaunig, Heather M. Smith, Paul Cornwell, Ruth A. Sanders

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Earlier work describing increased biliary excretion of the acetaminophen-cysteine conjugate advanced the hypothesis that streptozotocin-induced diabetes increases γ-glutamyltranspeptidase (GGT) expression in Sprague-Dawley rats. To test this hypothesis, rats were divided into control, diabetic, and insulin-treated diabetic groups. Diabetes was induced by intravenous injection of 45 mg streptozotocin/kg body weight and was effectively controlled by insulin treatment in the appropriate group. Densitometric quantification demonstrated that hepatic GGT activity in diabetic rats was significantly increased when compared to normal and insulin-treated diabetic controls. Histochemical staining of liver was greater in female than in male rats, and staining increased in female rat liver as the duration of diabetes lengthened from 30 to 90 days. GGT activity was increased by diabetes in liver canalicular-enriched and basolateral-enriched membrane preparations, and it was unchanged in renal brush border-enriched membranes. Total mRNA isolated from diabetic and insulin-treated diabetic rat livers did not conclusively demonstrate an elevation of GGT mRNA relative to normal. Western blot analysis showed no differences in the amount of GGT in diabetic versus normal rat livers. These data indicate that streptozotocin-induced diabetes does not alter the expression of, but does increase the activity of, GGT in liver.

Original languageEnglish (US)
Pages (from-to)219-225
Number of pages7
JournalJournal of Biochemical and Molecular Toxicology
Volume12
Issue number4
DOIs
StatePublished - Jan 1 1998

Fingerprint

Experimental Diabetes Mellitus
Streptozocin
Medical problems
Liver
Rats
Insulin
Staining and Labeling
Membranes
Messenger RNA
Brushes
Microvilli
Intravenous Injections
Sprague Dawley Rats
Western Blotting
Body Weight
Kidney

Keywords

  • Gamma-Glutamyltranspeptidase
  • Insulin-Dependent Diabetes Mellitus
  • Liver
  • Rats
  • Streptozotocin

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Health, Toxicology and Mutagenesis
  • Toxicology

Cite this

Streptozotocin-Induced Diabetes Increases γ-Glutamyltranspeptidase Activity but not Expression in Rat Liver. / Watkins, John B.; Klaunig, James E.; Smith, Heather M.; Cornwell, Paul; Sanders, Ruth A.

In: Journal of Biochemical and Molecular Toxicology, Vol. 12, No. 4, 01.01.1998, p. 219-225.

Research output: Contribution to journalArticle

Watkins, John B. ; Klaunig, James E. ; Smith, Heather M. ; Cornwell, Paul ; Sanders, Ruth A. / Streptozotocin-Induced Diabetes Increases γ-Glutamyltranspeptidase Activity but not Expression in Rat Liver. In: Journal of Biochemical and Molecular Toxicology. 1998 ; Vol. 12, No. 4. pp. 219-225.
@article{fdb21475211d4eb8bc15bdb6015c2702,
title = "Streptozotocin-Induced Diabetes Increases γ-Glutamyltranspeptidase Activity but not Expression in Rat Liver",
abstract = "Earlier work describing increased biliary excretion of the acetaminophen-cysteine conjugate advanced the hypothesis that streptozotocin-induced diabetes increases γ-glutamyltranspeptidase (GGT) expression in Sprague-Dawley rats. To test this hypothesis, rats were divided into control, diabetic, and insulin-treated diabetic groups. Diabetes was induced by intravenous injection of 45 mg streptozotocin/kg body weight and was effectively controlled by insulin treatment in the appropriate group. Densitometric quantification demonstrated that hepatic GGT activity in diabetic rats was significantly increased when compared to normal and insulin-treated diabetic controls. Histochemical staining of liver was greater in female than in male rats, and staining increased in female rat liver as the duration of diabetes lengthened from 30 to 90 days. GGT activity was increased by diabetes in liver canalicular-enriched and basolateral-enriched membrane preparations, and it was unchanged in renal brush border-enriched membranes. Total mRNA isolated from diabetic and insulin-treated diabetic rat livers did not conclusively demonstrate an elevation of GGT mRNA relative to normal. Western blot analysis showed no differences in the amount of GGT in diabetic versus normal rat livers. These data indicate that streptozotocin-induced diabetes does not alter the expression of, but does increase the activity of, GGT in liver.",
keywords = "Gamma-Glutamyltranspeptidase, Insulin-Dependent Diabetes Mellitus, Liver, Rats, Streptozotocin",
author = "Watkins, {John B.} and Klaunig, {James E.} and Smith, {Heather M.} and Paul Cornwell and Sanders, {Ruth A.}",
year = "1998",
month = "1",
day = "1",
doi = "10.1002/(SICI)1099-0461(1998)12:4<219::AID-JBT4>3.0.CO;2-O",
language = "English (US)",
volume = "12",
pages = "219--225",
journal = "Journal of Biochemical and Molecular Toxicology",
issn = "1095-6670",
publisher = "John Wiley and Sons Inc.",
number = "4",

}

TY - JOUR

T1 - Streptozotocin-Induced Diabetes Increases γ-Glutamyltranspeptidase Activity but not Expression in Rat Liver

AU - Watkins, John B.

AU - Klaunig, James E.

AU - Smith, Heather M.

AU - Cornwell, Paul

AU - Sanders, Ruth A.

PY - 1998/1/1

Y1 - 1998/1/1

N2 - Earlier work describing increased biliary excretion of the acetaminophen-cysteine conjugate advanced the hypothesis that streptozotocin-induced diabetes increases γ-glutamyltranspeptidase (GGT) expression in Sprague-Dawley rats. To test this hypothesis, rats were divided into control, diabetic, and insulin-treated diabetic groups. Diabetes was induced by intravenous injection of 45 mg streptozotocin/kg body weight and was effectively controlled by insulin treatment in the appropriate group. Densitometric quantification demonstrated that hepatic GGT activity in diabetic rats was significantly increased when compared to normal and insulin-treated diabetic controls. Histochemical staining of liver was greater in female than in male rats, and staining increased in female rat liver as the duration of diabetes lengthened from 30 to 90 days. GGT activity was increased by diabetes in liver canalicular-enriched and basolateral-enriched membrane preparations, and it was unchanged in renal brush border-enriched membranes. Total mRNA isolated from diabetic and insulin-treated diabetic rat livers did not conclusively demonstrate an elevation of GGT mRNA relative to normal. Western blot analysis showed no differences in the amount of GGT in diabetic versus normal rat livers. These data indicate that streptozotocin-induced diabetes does not alter the expression of, but does increase the activity of, GGT in liver.

AB - Earlier work describing increased biliary excretion of the acetaminophen-cysteine conjugate advanced the hypothesis that streptozotocin-induced diabetes increases γ-glutamyltranspeptidase (GGT) expression in Sprague-Dawley rats. To test this hypothesis, rats were divided into control, diabetic, and insulin-treated diabetic groups. Diabetes was induced by intravenous injection of 45 mg streptozotocin/kg body weight and was effectively controlled by insulin treatment in the appropriate group. Densitometric quantification demonstrated that hepatic GGT activity in diabetic rats was significantly increased when compared to normal and insulin-treated diabetic controls. Histochemical staining of liver was greater in female than in male rats, and staining increased in female rat liver as the duration of diabetes lengthened from 30 to 90 days. GGT activity was increased by diabetes in liver canalicular-enriched and basolateral-enriched membrane preparations, and it was unchanged in renal brush border-enriched membranes. Total mRNA isolated from diabetic and insulin-treated diabetic rat livers did not conclusively demonstrate an elevation of GGT mRNA relative to normal. Western blot analysis showed no differences in the amount of GGT in diabetic versus normal rat livers. These data indicate that streptozotocin-induced diabetes does not alter the expression of, but does increase the activity of, GGT in liver.

KW - Gamma-Glutamyltranspeptidase

KW - Insulin-Dependent Diabetes Mellitus

KW - Liver

KW - Rats

KW - Streptozotocin

UR - http://www.scopus.com/inward/record.url?scp=0031617109&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0031617109&partnerID=8YFLogxK

U2 - 10.1002/(SICI)1099-0461(1998)12:4<219::AID-JBT4>3.0.CO;2-O

DO - 10.1002/(SICI)1099-0461(1998)12:4<219::AID-JBT4>3.0.CO;2-O

M3 - Article

C2 - 9580874

AN - SCOPUS:0031617109

VL - 12

SP - 219

EP - 225

JO - Journal of Biochemical and Molecular Toxicology

JF - Journal of Biochemical and Molecular Toxicology

SN - 1095-6670

IS - 4

ER -