Streptozotocin May Provide Protection against Subsequent Oxidative Stress of Endotoxin or Streptozotocin in Rats

Hossam M. Omar, Jason K. Rosenblum, Ruth A. Sanders, John B. Watkins

Research output: Contribution to journalArticle

3 Scopus citations

Abstract

Endotoxin lipopolysaccharide (LPS) and streptozotocin-induced diabetes are known to cause oxidative stress in vivo. There is some evidence that a sublethal dose of LPS provides protection against subsequent oxidative stress. Because of its wide use as a diabetogenic agent, this study was undertaken to determine if streptozotocin can likewise provide a protective effect against further oxidative stress in rats. Female Sprague-Dawley rats were given streptozotocin (50 mg/kg intraperitoneally once) prior to exposure to either bacterial endotoxin from Salmonella abortus equii (5 mg/kg intraperitoneally) or three additional daily doses of streptozotocin (50 mg/kg intraperitoneally). One week after LPS or streptozotocin treatments, oxidative stress was determined by measuring changes in antioxidant activity (glutathione peroxidase, glutathione reductase, superoxide dismutase, catalase, glutathione S-transferase, and γ-glutamyltranspeptidase) and in concentrations of glutathione, nitrite, and thiobarbituric acid reactants in liver, kidney, intestine, and spleen. High levels of some antioxidants in the LPS-control and streptozotocin-control rats, in contrast to normal levels found in diabetes + LPS and multidose-streptozotocin rats, suggest that streptozotocin, like LPS, may confer a protective effect against subsequent oxidative stress.

Original languageEnglish (US)
Pages (from-to)143-149
Number of pages7
JournalJournal of Biochemical and Molecular Toxicology
Volume12
Issue number3
DOIs
StatePublished - Jan 1 1998

Keywords

  • Anti-oxidant
  • Diabetes
  • Endotoxin
  • Free Radical
  • Lipid Peroxidation
  • Lipopolysaccharide
  • Oxidative Stress
  • Rat
  • Streptozotocin

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Health, Toxicology and Mutagenesis
  • Toxicology

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