Structural and functional characteristics and tissue distribution pattern of rat OCTN1, an organic cation transporter, cloned from placenta

Xiang Wu, Ronald L. George, Wei Huang, Haiping Wang, Simon Conway, Frederick H. Leibach, Vadivel Ganapathy

Research output: Contribution to journalArticle

163 Citations (Scopus)

Abstract

This report describes the structure, function, and tissue distribution pattern of rat OCTN1 (novel organic cation transporter 1). The rat OCTN1 cDNA was isolated from a rat placental cDNA library. The cDNA is 2258 bp long and codes for a protein of 553 amino acids. Its amino acid sequence bears high homology to human OCTN1 (85% identity) and rat OCTN2 (74% identity). When expressed heterologously in mammalian cells, rat OCTN1 mediates Na+-independent and pH-dependent transport of the prototypical organic cation tetraethylammonium. The transporter interacts with a variety of structurally diverse organic cations such as desipramine, dimethylamiloride, cimetidine, procainamide, and verapamil. Carnitine, a zwitterion, interacts with rat OCTN1 with a very low affinity. However, the transport of carnitine via rat OCTN1 is not evident in the presence or absence of Na+. We conclude that rat OCTN1 is a multispecific organic cation transporter. OCTN1-specific mRNA transcripts are present in a wide variety of tissues in the rat, principally in the liver, intestine, kidney, brain, heart and placenta. In situ hybridization shows the distribution pattern of the transcripts in the brain (cerebellum, hippocampus and cortex), kidney (cortex and medulla with relatively more abundance in the cortical-medullary junction), heart (myocardium and valves) and placenta (labyrinthine zone). Copyright (C) 2000 Elsevier Science B.V.

Original languageEnglish (US)
Pages (from-to)315-327
Number of pages13
JournalBiochimica et Biophysica Acta - Biomembranes
Volume1466
Issue number1-2
DOIs
StatePublished - Jun 1 2000
Externally publishedYes

Fingerprint

Organic Cation Transporter 1
Tissue Distribution
Placenta
Cations
Rats
Tissue
Carnitine
Complementary DNA
Kidney Medulla
Procainamide
Kidney Cortex
Desipramine
Tetraethylammonium
Brain
Cimetidine
Heart Valves
Verapamil
Gene Library
Cerebellum
Intestines

Keywords

  • Carnitine
  • Drug disposition
  • In situ hybridization
  • OCTN1
  • Organic cation transport
  • Rat

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Biophysics

Cite this

Structural and functional characteristics and tissue distribution pattern of rat OCTN1, an organic cation transporter, cloned from placenta. / Wu, Xiang; George, Ronald L.; Huang, Wei; Wang, Haiping; Conway, Simon; Leibach, Frederick H.; Ganapathy, Vadivel.

In: Biochimica et Biophysica Acta - Biomembranes, Vol. 1466, No. 1-2, 01.06.2000, p. 315-327.

Research output: Contribution to journalArticle

Wu, Xiang ; George, Ronald L. ; Huang, Wei ; Wang, Haiping ; Conway, Simon ; Leibach, Frederick H. ; Ganapathy, Vadivel. / Structural and functional characteristics and tissue distribution pattern of rat OCTN1, an organic cation transporter, cloned from placenta. In: Biochimica et Biophysica Acta - Biomembranes. 2000 ; Vol. 1466, No. 1-2. pp. 315-327.
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