Structural and functional comparison of a 22 kDa protein from internal human platelet membranes with cardiac phospholamban

Samuel Evans Adunyah, Larry Jones, william L. Dean

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

We have shown that a platelet 22 kDa phosphoprotein is similar to the cardiac regulator phospholamban, in that both are phosphorylated by cAMP- and calmodulin-dependent protein kinases, and that both can be phosphorylated simultaneously by these two classes of protein kinases to yield an additive stimulation of the respective Ca2+ pumps (Adunyah, S.E. and Dean, W.L. (1987) Biochim. Biophys. Acta 930, 401-409). However, whereas phosphorylation of phospholamban increases the affinity of the cardiac Ca2+-ATPase for Ca2+, phosphorylation of the platelet 22 kDa protein increased the Vmax of the pump. In addition, antibodies raised against canine phospholamban did not crossreact with the human platelet 22 kDa protein. Finally, it was not possible to dissociate the platelet protein into lower-molecular-weight subunits by boiling in sodium dodecylsulfate which is characteristic of cardiac phospholamban. These results show that although phosphorylation of low-molecular-weight membrane-associated regulator proteins in cardiac muscle and platelets appears to stimulate the respective Ca2+ pumps, these proteins have different chemical and physical properties.

Original languageEnglish
Pages (from-to)63-70
Number of pages8
JournalBiochimica et Biophysica Acta - Biomembranes
Volume941
Issue number1
DOIs
StatePublished - Jun 7 1988

Fingerprint

Platelets
Blood Platelets
Phosphorylation
Membranes
Pumps
Proteins
Molecular Weight
Molecular weight
Calcium-Calmodulin-Dependent Protein Kinases
Calcium-Transporting ATPases
Phosphoproteins
Cyclic AMP-Dependent Protein Kinases
Boiling liquids
Protein Kinases
Chemical properties
Muscle
Canidae
Myocardium
Membrane Proteins
Physical properties

Keywords

  • ATPase, Ca-
  • cyclic AMP
  • Phospholamban
  • Platelet
  • Protein, 22 kDa

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Cell Biology

Cite this

Structural and functional comparison of a 22 kDa protein from internal human platelet membranes with cardiac phospholamban. / Adunyah, Samuel Evans; Jones, Larry; Dean, william L.

In: Biochimica et Biophysica Acta - Biomembranes, Vol. 941, No. 1, 07.06.1988, p. 63-70.

Research output: Contribution to journalArticle

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