Structural changes in transthyretin produced by the Ile 84 Ser mutation which result in decreased affinity for retinol-binding protein

Jean A. Hamilton, Larry K. Steinrauf, Bradford C. Braden, Jill R. Murrell, Merrill Benson

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

The X-ray crystal structure of the transthyretin variant Ile 84 Ser has been determined to 1.7Å resolution. The functional form of normal transthyretin is a tetramer, having a cylindrical cavity which binds thyroxine and an exterior binding site for the complex of retinol with retinol-binding protein. The naturally occurring amyloidogenic variant Ile 84 Ser has reduced affinity for retinol-binding protein. The results of this study show that the overall structural homology between the C-alpha atoms of the variant and normal protein is very high with differences mainly in the loop regions, in the vicinity of the substitution, and the ligand binding areas. The known functional differences for the variant (ligand-binding) are correlated with the structural changes observed in the region of the ligand-binding site. The substitution at position 84 results in changes in the shape of the putative site for complexation with retinol binding protein. The substitution site occurs at the interface between two tetramers which are nearest neighbors in the crystal in the direction parallel to the a axis of the unit cell. The variant tetramers move closer together resulting in a hydrogen bond across the interface which does not form in the normal protein. This dominant packing interface may be significant in amyloidogenesis.

Original languageEnglish
Pages (from-to)1-12
Number of pages12
JournalAmyloid
Volume3
Issue number1
StatePublished - 1996

Fingerprint

Retinol-Binding Proteins
Prealbumin
Ligands
Mutation
Binding Sites
Vitamin A
Thyroxine
Hydrogen
Proteins
X-Rays

Keywords

  • Amyloidosis
  • Ile 84 Ser mutation
  • Retinol-binding protein
  • Transthyretin

ASJC Scopus subject areas

  • Medicine(all)
  • Pathology and Forensic Medicine

Cite this

Structural changes in transthyretin produced by the Ile 84 Ser mutation which result in decreased affinity for retinol-binding protein. / Hamilton, Jean A.; Steinrauf, Larry K.; Braden, Bradford C.; Murrell, Jill R.; Benson, Merrill.

In: Amyloid, Vol. 3, No. 1, 1996, p. 1-12.

Research output: Contribution to journalArticle

Hamilton, Jean A. ; Steinrauf, Larry K. ; Braden, Bradford C. ; Murrell, Jill R. ; Benson, Merrill. / Structural changes in transthyretin produced by the Ile 84 Ser mutation which result in decreased affinity for retinol-binding protein. In: Amyloid. 1996 ; Vol. 3, No. 1. pp. 1-12.
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