Structural changes induced by osmotic water flow in rabbit proximal tubule

James Williams, Dale R. Abrahamson, James A. Schafer

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

When a transepithelial osmotic difference was imposed in perfused proximal straight tubules (270 mOsm/kg H2O in the lumen and 290 in the bath) in the absence of bath colloid, a severe vacuolation (appearance of lucent spaces) developed within the epithelium such that view of the lumen border was obscured within 5 ± 1 min (N = 13 tubules at 23°C). This vacuolation was less severe if the bath was hypotonie to the lumen or if the magnitude of the osmotic difference was reduced. If colloid (6% wt/vol of either bovine serum albumin or 70,000 molecular wt dextran) was included in the bathing medium, vacuolation was either not observed or was minimal, but became severe upon removal of the colloid and obscured the lumen within 6 ± 1 min (N = 8 for albumin and N = 4 for dextran at 23°C). At 38°C, vacuolation obscured the lumen within 4 ± 1 min following the removal of albumin (N = 5). ANOVA suggests that none of the times for vacuolation to occur differed. The rate of passive volume flow due to the osmotic difference was unaffected by vacuolation (0.9 ± 0.1 nl · min-1 · mm-1 with albumin to 0.8 ± 0.1 without albumin and vacuolated, N = 8 at 23°C, P > 0.2 using a paired t-test). Electron microscopic examination of tubules fixed after vacuolation showed lucent spaces within the cytoplasm. These results suggest that the presence of serosal colloid protected the epithelial cells from injury during rapid transepithelial water flow. The mechanism for this protective effect is not apparent, but may be related to effects of colloid in maintaining normal volume absorption in the proximal nephron.

Original languageEnglish (US)
Pages (from-to)672-683
Number of pages12
JournalKidney International
Volume39
Issue number4
StatePublished - Apr 1991
Externally publishedYes

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Colloids
Albumins
Rabbits
Baths
Water
Dextrans
Nephrons
Bovine Serum Albumin
Analysis of Variance
Cytoplasm
Epithelium
Epithelial Cells
Electrons
Wounds and Injuries

ASJC Scopus subject areas

  • Nephrology

Cite this

Structural changes induced by osmotic water flow in rabbit proximal tubule. / Williams, James; Abrahamson, Dale R.; Schafer, James A.

In: Kidney International, Vol. 39, No. 4, 04.1991, p. 672-683.

Research output: Contribution to journalArticle

Williams, J, Abrahamson, DR & Schafer, JA 1991, 'Structural changes induced by osmotic water flow in rabbit proximal tubule', Kidney International, vol. 39, no. 4, pp. 672-683.
Williams, James ; Abrahamson, Dale R. ; Schafer, James A. / Structural changes induced by osmotic water flow in rabbit proximal tubule. In: Kidney International. 1991 ; Vol. 39, No. 4. pp. 672-683.
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