Structural correlates for enhanced stability in the E2 DNA-binding domain from bovine papillomavirus

Sudha Veeraraghavan, Cecilia C. Mello, Elliot Androphy, James D. Baleja

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Papillomaviral E2 proteins participate in viral DNA replication and transcriptional regulation. We have solved the solution structure of the DNA- binding domain of the E2 protein from bovine papillomavirus (BPV-1). The structure calculation used 2222 distance and 158 dihedral angle restraints for the homodimer (202 residues in total), which were derived from homonuclear and heteronuclear multidimensional nuclear magnetic resonance (NMR) spectroscopic data. The root-mean-square deviation for structured regions of the monomer when superimposed to the average is 0.73 ± 0.10 Å for backbone atoms and 1.42 ± 0.16 Å for heavy atoms. The 101 residue construct used in this study (residues 310410) is about 4.5 kcal/mol more stable than a minimal domain comprising the C-terminal 85 amino acid residues (residues 326-410). The structure of the core domain contained within BPV-1 E2 is similar to the corresponding regions of other papillomaviral E2 proteins. Here, however, the extra N-terminal 16 residues form a flap that covers a cavity at the dimer interface and play a role in DNA binding. Interactions between residues in the N-terminal extension and the core domain correlate with the greater stability of the longer form of the protein relative to the minimal domain.

Original languageEnglish (US)
Pages (from-to)16115-16124
Number of pages10
JournalBiochemistry
Volume38
Issue number49
DOIs
StatePublished - Dec 7 1999
Externally publishedYes

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DNA
Biomolecular Nuclear Magnetic Resonance
Atoms
Proteins
Viral DNA
Dihedral angle
DNA Replication
Dimers
Monomers
Nuclear magnetic resonance
Amino Acids
Bovine papillomavirus E2 protein

ASJC Scopus subject areas

  • Biochemistry

Cite this

Structural correlates for enhanced stability in the E2 DNA-binding domain from bovine papillomavirus. / Veeraraghavan, Sudha; Mello, Cecilia C.; Androphy, Elliot; Baleja, James D.

In: Biochemistry, Vol. 38, No. 49, 07.12.1999, p. 16115-16124.

Research output: Contribution to journalArticle

Veeraraghavan, Sudha ; Mello, Cecilia C. ; Androphy, Elliot ; Baleja, James D. / Structural correlates for enhanced stability in the E2 DNA-binding domain from bovine papillomavirus. In: Biochemistry. 1999 ; Vol. 38, No. 49. pp. 16115-16124.
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