Structure and Biophysics for a Six Letter DNA Alphabet that Includes Imidazo[1,2-a]-1,3,5-triazine-2(8H)-4(3H)-dione (X) and 2,4-Diaminopyrimidine (K)

Isha Singh, Myong Jung Kim, Robert W. Molt, Shuichi Hoshika, Steven A. Benner, Millie Georgiadis

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

A goal of synthetic biology is to develop new nucleobases that retain the desirable properties of natural nucleobases at the same time as expanding the genetic alphabet. The nonstandard Watson-Crick pair between imidazo[1,2-a]-1,3,5-triazine-2(8H)-4(3H)-dione (X) and 2,4-diaminopyrimidine (K) does exactly this, pairing via complementary arrangements of hydrogen bonding in these two nucleobases, which do not complement any natural nucleobase. Here, we report the crystal structure of a duplex DNA oligonucleotide in B-form including two consecutive X:K pairs in GATCXK DNA determined as a host-guest complex at 1.75 Å resolution. X:K pairs have significant propeller twist angles, similar to those observed for A:T pairs, and a calculated hydrogen bonding pairing energy that is weaker than that of A:T. Thus, although inclusion of X:K pairs results in a duplex DNA structure that is globally similar to that of an analogous G:C structure, the X:K pairs locally and energetically more closely resemble A:T pairs.

Original languageEnglish (US)
Pages (from-to)2118-2129
Number of pages12
JournalACS Synthetic Biology
Volume6
Issue number11
DOIs
StatePublished - Nov 17 2017

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Biophysics
Triazines
DNA
Hydrogen Bonding
Hydrogen bonds
Synthetic Biology
Oligonucleotides
Propellers
Crystal structure
2,4-diaminopyrimidine

ASJC Scopus subject areas

  • Biomedical Engineering
  • Biochemistry, Genetics and Molecular Biology (miscellaneous)

Cite this

Structure and Biophysics for a Six Letter DNA Alphabet that Includes Imidazo[1,2-a]-1,3,5-triazine-2(8H)-4(3H)-dione (X) and 2,4-Diaminopyrimidine (K). / Singh, Isha; Kim, Myong Jung; Molt, Robert W.; Hoshika, Shuichi; Benner, Steven A.; Georgiadis, Millie.

In: ACS Synthetic Biology, Vol. 6, No. 11, 17.11.2017, p. 2118-2129.

Research output: Contribution to journalArticle

Singh, Isha ; Kim, Myong Jung ; Molt, Robert W. ; Hoshika, Shuichi ; Benner, Steven A. ; Georgiadis, Millie. / Structure and Biophysics for a Six Letter DNA Alphabet that Includes Imidazo[1,2-a]-1,3,5-triazine-2(8H)-4(3H)-dione (X) and 2,4-Diaminopyrimidine (K). In: ACS Synthetic Biology. 2017 ; Vol. 6, No. 11. pp. 2118-2129.
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