Structure and Function of a Long Alternating Purine-Pyrimidine Sequence in the Mouse Alcohol Dehydrogenase Adh-1 Gene

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Abstract

The mouse alcohol dehydrogenase gene Adh-1 contains an unusually long alternating purine-pyrimidine sequence within its first intron. This alternating sequence differs in length between strains that differ in the extent of Adh-1 expression, and it has been suggested that it plays a role in gene expression. We demonstrate that this alternating sequence can form Z-DNA in vitro. The alternating sequence can act as a positive regulatory element in transient transfection assays in hepatoma cell lines, but not in CV-1 (monkey kidney) cells, suggesting that it can act as a tissue-specific regulatory element. Nuclear run-on experiments showed that the differential expression of Adh-1 from high- and low-activity strains is, however, controlled at the post-transcription level.

Original languageEnglish
Pages (from-to)407-412
Number of pages6
JournalArchives of Biochemistry and Biophysics
Volume316
Issue number1
DOIs
StatePublished - Jan 1995

Fingerprint

Z-Form DNA
Alcohol Dehydrogenase
Introns
Haplorhini
Transfection
Hepatocellular Carcinoma
Genes
Kidney
Gene Expression
Cell Line
Transcription
Gene expression
Assays
Cells
Tissue
Experiments
purine
In Vitro Techniques
1,2-diamino-1,2-N,N'-carbonyl-1,2-dideoxyglucose hydrate
pyrimidine

ASJC Scopus subject areas

  • Molecular Biology
  • Biophysics
  • Biochemistry

Cite this

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AB - The mouse alcohol dehydrogenase gene Adh-1 contains an unusually long alternating purine-pyrimidine sequence within its first intron. This alternating sequence differs in length between strains that differ in the extent of Adh-1 expression, and it has been suggested that it plays a role in gene expression. We demonstrate that this alternating sequence can form Z-DNA in vitro. The alternating sequence can act as a positive regulatory element in transient transfection assays in hepatoma cell lines, but not in CV-1 (monkey kidney) cells, suggesting that it can act as a tissue-specific regulatory element. Nuclear run-on experiments showed that the differential expression of Adh-1 from high- and low-activity strains is, however, controlled at the post-transcription level.

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