Structure-function analysis of GNIP, the glycogenin-interacting protein

Lanmin Zhai, Amy Dietrich, Alexander Skurat, Peter Roach

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Glycogenin is a self-glucosylating protein that initiates glycogen biosynthesis. We recently identified a family of proteins, GNIPs, that interact with glycogenin and stimulate its self-glucosylating activity [J. Biol. Chem. 277 (2002) 19331]. The GNIP gene (also called TRIM7) encodes at least four distinct isoforms of GNIP, three of which (GNIP1, GNIP2, and GNIP3) have in common a COOH-terminal B30.2 domain and predicted coiled-coil regions. Based on Western blot analysis, the GNIP1 protein is widely distributed in tissues. From analysis of a series of deletion mutants of GNIP2 using the yeast two-hybrid system, the B30.2 domain was found to be responsible for the interaction with glycogenin. A truncated form of recombinant GNIP2, lacking the NH 2-terminal coiled-coil region, was cross-linked to glycogenin by glutaraldehyde treatment, supporting the idea that the B30.2 domain was sufficient for the interaction. In the course of this study, GNIP2 was also found to interact with itself, via the coiled-coil domain. Heterologous interactions between GNIP1 and GNIP2 were also detected. Since glycogenin is also a dimer, higher order multimeric complexes between glycogenin and GNIPs would be possible.

Original languageEnglish
Pages (from-to)236-242
Number of pages7
JournalArchives of Biochemistry and Biophysics
Volume421
Issue number2
DOIs
StatePublished - Jan 15 2004

Fingerprint

Proteins
Two-Hybrid System Techniques
Biosynthesis
Glutaral
Hybrid systems
Glycogen
Dimers
Yeast
glycogenin
Protein Isoforms
Genes
Western Blotting
Tissue
B30.2-SPRY Domain

Keywords

  • B30.2
  • Coiled-coil
  • Glycogen
  • Glycogenin
  • TRIM7

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Structure-function analysis of GNIP, the glycogenin-interacting protein. / Zhai, Lanmin; Dietrich, Amy; Skurat, Alexander; Roach, Peter.

In: Archives of Biochemistry and Biophysics, Vol. 421, No. 2, 15.01.2004, p. 236-242.

Research output: Contribution to journalArticle

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