Structure of the human liver/bone/kidney alkaline phosphatase gene

M. J. Weiss, K. Ray, P. S. Henthorn, B. Lamb, T. Kadesch, H. Harris

Research output: Contribution to journalArticle

263 Scopus citations

Abstract

In man, there are multiple forms of alkaline phosphatase encoded by at least three homologous genes: placental, intestinal, and liver/bone/kidney. This report describes the characterization of the human liver/bone/kidney alkaline phosphatase locus. The gene appears to exist as a single copy in the haploid genome and is comprised of 12 exons distributed over more than 50 kilobases. In liver, kidney, SAOS-2 human osteosarcoma cells, and cultured fibroblasts, there is a single major start for transcription situated about 25 nucleotides downstream of an A/T-rich motif. The promoter region is extremely G/C-rich, is relatively abundant in the dinucleotide CpG, and contains four copies of the consensus sequence for SP1 binding (GGGCGG). The liver/bone/kidney alkaline phosphatase gene is at least five times larger than the intestinal and placental alkaline phsophatase genes, mainly due to intron size differences. Intron-exon junctions occur at analogous positions in all three genes, but there is an extra noncoding exon at the 5' end of the liver/bone/kidney alkaline phosphatase gene. The relevance of our findings with respect to the evolution of the human alkaline phosphatase multigene family is discussed.

Original languageEnglish (US)
Pages (from-to)12002-12010
Number of pages9
JournalJournal of Biological Chemistry
Volume263
Issue number24
StatePublished - Jan 1 1988
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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  • Cite this

    Weiss, M. J., Ray, K., Henthorn, P. S., Lamb, B., Kadesch, T., & Harris, H. (1988). Structure of the human liver/bone/kidney alkaline phosphatase gene. Journal of Biological Chemistry, 263(24), 12002-12010.