Structure of the human liver/bone/kidney alkaline phosphatase gene

M. J. Weiss, K. Ray, P. S. Henthorn, Bruce Lamb, T. Kadesch, H. Harris

Research output: Contribution to journalArticle

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Abstract

In man, there are multiple forms of alkaline phosphatase encoded by at least three homologous genes: placental, intestinal, and liver/bone/kidney. This report describes the characterization of the human liver/bone/kidney alkaline phosphatase locus. The gene appears to exist as a single copy in the haploid genome and is comprised of 12 exons distributed over more than 50 kilobases. In liver, kidney, SAOS-2 human osteosarcoma cells, and cultured fibroblasts, there is a single major start for transcription situated about 25 nucleotides downstream of an A/T-rich motif. The promoter region is extremely G/C-rich, is relatively abundant in the dinucleotide CpG, and contains four copies of the consensus sequence for SP1 binding (GGGCGG). The liver/bone/kidney alkaline phosphatase gene is at least five times larger than the intestinal and placental alkaline phsophatase genes, mainly due to intron size differences. Intron-exon junctions occur at analogous positions in all three genes, but there is an extra noncoding exon at the 5' end of the liver/bone/kidney alkaline phosphatase gene. The relevance of our findings with respect to the evolution of the human alkaline phosphatase multigene family is discussed.

Original languageEnglish (US)
Pages (from-to)12002-12010
Number of pages9
JournalJournal of Biological Chemistry
Volume263
Issue number24
StatePublished - 1988
Externally publishedYes

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Genes
Alkaline Phosphatase
Liver
Kidney
Exons
Bone
Bone and Bones
Introns
Haploidy
Consensus Sequence
Osteosarcoma
Multigene Family
Genetic Promoter Regions
Transcription
Fibroblasts
human ALPL protein
Cultured Cells
Nucleotides
Genome

ASJC Scopus subject areas

  • Biochemistry

Cite this

Weiss, M. J., Ray, K., Henthorn, P. S., Lamb, B., Kadesch, T., & Harris, H. (1988). Structure of the human liver/bone/kidney alkaline phosphatase gene. Journal of Biological Chemistry, 263(24), 12002-12010.

Structure of the human liver/bone/kidney alkaline phosphatase gene. / Weiss, M. J.; Ray, K.; Henthorn, P. S.; Lamb, Bruce; Kadesch, T.; Harris, H.

In: Journal of Biological Chemistry, Vol. 263, No. 24, 1988, p. 12002-12010.

Research output: Contribution to journalArticle

Weiss, MJ, Ray, K, Henthorn, PS, Lamb, B, Kadesch, T & Harris, H 1988, 'Structure of the human liver/bone/kidney alkaline phosphatase gene', Journal of Biological Chemistry, vol. 263, no. 24, pp. 12002-12010.
Weiss MJ, Ray K, Henthorn PS, Lamb B, Kadesch T, Harris H. Structure of the human liver/bone/kidney alkaline phosphatase gene. Journal of Biological Chemistry. 1988;263(24):12002-12010.
Weiss, M. J. ; Ray, K. ; Henthorn, P. S. ; Lamb, Bruce ; Kadesch, T. ; Harris, H. / Structure of the human liver/bone/kidney alkaline phosphatase gene. In: Journal of Biological Chemistry. 1988 ; Vol. 263, No. 24. pp. 12002-12010.
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