Studies of renal injury. II. Activation of the glucose transporter 1 (GLUT1) gene and glycolysis in LLC-PK1 cells under Ca2+ stress

Jesus Dominguez, Buguang Song, Shuxian Liu-Chen, Mona Qulali, Randy Howard, Chao-Hung Lee, James McAteer

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Injury to the renal proximal tubule is common and may be followed by either recovery or cell death. The survival of injured cells is supported by a transient change in cellular metabolism that maintains life even when oxygen tension is reduced. This adaptive process involves the activation of the gene encoding the glucose transporter GLUT1, which is essential to maintain the high rates of glucose influx demanded by glycolysis. We hypothesized that after cell injury increases of cell Ca2+ (Ca2+(i)) initiate the flow of information that culminates with the upregulation of the stress response gene GLUT1. We found that elevations of Ca2+(i) caused by the calcium ionophore A23187 activated the expression of the GLUT1 gene in LLC-PK1 cells. The stimulatory effect of Ca2+(i) on GLUT1 gene expression was, at least in part, transcriptional and resulted in higher levels of GLUT1 mRNA, cognate protein, cellular hexose transport activity, glucose consumption, and lactate production. This response was vital to the renal cells, as its interruption severely increased Ca2+-induced cytotoxicity and cell mortality. We propose that increases of Ca2+(i) initiate stress responses, represented in part by activation of the GLUT1 gene, and that disruption to the flow of information originating from Ca2+-induced stress, or to the coordinated expression of the stress response, prevents cell recovery after injury and may be an important cause of permanent renal cell injury and cell death.

Original languageEnglish
Pages (from-to)395-404
Number of pages10
JournalJournal of Clinical Investigation
Volume98
Issue number2
StatePublished - Jul 15 1996

Fingerprint

LLC-PK1 Cells
Facilitative Glucose Transport Proteins
Glycolysis
Kidney
Wounds and Injuries
Genes
Cell Death
Monosaccharide Transport Proteins
Glucose
Proximal Kidney Tubule
Calcium Ionophores
Calcimycin
Transcriptional Activation
Lactic Acid
Cell Survival
Up-Regulation
Oxygen
Gene Expression
Messenger RNA
Mortality

Keywords

  • acute renal failure
  • cytosolic calcium
  • glucose transport
  • renal tubule

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Studies of renal injury. II. Activation of the glucose transporter 1 (GLUT1) gene and glycolysis in LLC-PK1 cells under Ca2+ stress. / Dominguez, Jesus; Song, Buguang; Liu-Chen, Shuxian; Qulali, Mona; Howard, Randy; Lee, Chao-Hung; McAteer, James.

In: Journal of Clinical Investigation, Vol. 98, No. 2, 15.07.1996, p. 395-404.

Research output: Contribution to journalArticle

Dominguez, Jesus ; Song, Buguang ; Liu-Chen, Shuxian ; Qulali, Mona ; Howard, Randy ; Lee, Chao-Hung ; McAteer, James. / Studies of renal injury. II. Activation of the glucose transporter 1 (GLUT1) gene and glycolysis in LLC-PK1 cells under Ca2+ stress. In: Journal of Clinical Investigation. 1996 ; Vol. 98, No. 2. pp. 395-404.
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