Studies of the functional and morphologic status of islets maintained at 24 C for four weeks in vitro

J. Ono, P. E. Lacy, H. E.B. Michael, M. H. Greider

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32 Scopus citations

Abstract

Isolated rat islets were maintained in vitro at 24 C for 1-4 wk in tissue culture medium containing D-glucose (1.5 mg/ml). The rate of insulin release at 24 C remained stable for 3 wk (2.2 μU/islet/hr) and decreased to 1.2 μU/islet/hr during the 4th wk. Increasing the temperature from 24 C to 37 C at the end of 1, 2, 3, or 4 wk produced a 5- to 7-fold increase in the rate of insulin release in the presence of glucose (1.5 mg/ml). This rate of secretion was comparable to control islets maintained at 37 C for 1-4 wk. Light- and electron-microscopic studies revealed minimal central necrosis of large islets maintained at 24 C for 3 wk. In contrast, extensive central necrosis was present in large islets maintained at 37 C for only 1 wk. Degranulation of B cells occurred at 24 C with almost complete degranulation at 28 days. Regranulation occurred when the temperature was increased to 37 C. These findings indicate that isolated islets maintained at 24 C remain functionally and morphologically intact for 4 islets Initial studies have shown that maintenance of ilsets at 24 C for 1 wk in conjunction with a single injection of antilymphocyte serum will produce marked prolongation of survival of islet allografts. The finding that isolated islets will survive for prolonged periods of time at 24 C should be of importance to future studies on islet transplantation, immune rejection, and investigations on hormonal release from islets maintained under these conditions.

Original languageEnglish (US)
Pages (from-to)489-504
Number of pages16
JournalAmerican Journal of Pathology
Volume97
Issue number3
StatePublished - Dec 1 1979

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

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