Studies on gene control regions X. The effect of specific adenine-thymine transversions on the lac repressor-lac operator interaction

H. S. Sista, R. T. Loder, M. H. Caruthers

Research output: Contribution to journalArticle

8 Scopus citations

Abstract

Chemical and enzymatic methods were used to synthesize a transition (AT to GC) and a transversion (AT to TA) at a lac operator site known to interact with lac repressor through the thymine 5 methyl group. These operators also contained a poly(dA)·poly(dT) tail 8 to 12 base pairs in length at one end. Results suggest that the steric constraints of lac repressor relative to the position of the 5 methyl group are quite critical. For example a seven fold reduction in stability was observed for the transversion. Results also suggest that the operator spans at least 21 base pairs.

Original languageEnglish (US)
Pages (from-to)2583-2599
Number of pages17
JournalNucleic acids research
Volume6
Issue number7
DOIs
StatePublished - Jun 11 1979
Externally publishedYes

ASJC Scopus subject areas

  • Genetics

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