Studies on gene control regions X. The effect of specific adenine-thymine transversions on the lac repressor-lac operator interaction

H. S. Sista, Randall Loder, M. H. Caruthers

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Chemical and enzymatic methods were used to synthesize a transition (AT to GC) and a transversion (AT to TA) at a lac operator site known to interact with lac repressor through the thymine 5 methyl group. These operators also contained a poly(dA)·poly(dT) tail 8 to 12 base pairs in length at one end. Results suggest that the steric constraints of lac repressor relative to the position of the 5 methyl group are quite critical. For example a seven fold reduction in stability was observed for the transversion. Results also suggest that the operator spans at least 21 base pairs.

Original languageEnglish (US)
Pages (from-to)2583-2599
Number of pages17
JournalNucleic Acids Research
Volume6
Issue number7
DOIs
StatePublished - Jun 11 1979
Externally publishedYes

Fingerprint

Lac Repressors
Thymine
Adenine
Base Pairing
Genes
Gene
Operator
Interaction
Tail
Fold

ASJC Scopus subject areas

  • Statistics, Probability and Uncertainty
  • Applied Mathematics
  • Health, Toxicology and Mutagenesis
  • Toxicology
  • Genetics(clinical)
  • Genetics

Cite this

Studies on gene control regions X. The effect of specific adenine-thymine transversions on the lac repressor-lac operator interaction. / Sista, H. S.; Loder, Randall; Caruthers, M. H.

In: Nucleic Acids Research, Vol. 6, No. 7, 11.06.1979, p. 2583-2599.

Research output: Contribution to journalArticle

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