Studies on the DNA elongation inhibitor and its proliferating cell nuclear antigen-dependent control in simian virus 40 DNA replication in vitro

Suk-Hee Lee, A. D. Kwong, Y. Ishimi, J. Hurwitz

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

A 120-kDa protein that blocks DNA termini has been purified from extracts of HeLa cells. This protein inhibits the action of a number of enzymes that catalyze reactions involving the 5' and 3' ends of DNA (DNA ligase, 3' and 5' exonucleases, and DNA polymerase α). The 120-kDa protein blocks the synthesis of long DNA chains that are normally formed during simian virus 40 DNA replication, causing the accumulation of small DNA fragments. The effects of this protein can be reversed by the addition of proliferating cell nuclear antigen and other protein fractions (activators).

Original languageEnglish (US)
Pages (from-to)4877-4881
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume86
Issue number13
StatePublished - 1989
Externally publishedYes

Fingerprint

Simian virus 40
Proliferating Cell Nuclear Antigen
DNA Replication
DNA
Proteins
DNA Ligases
Exonucleases
DNA-Directed DNA Polymerase
HeLa Cells
In Vitro Techniques
Enzymes

ASJC Scopus subject areas

  • General
  • Genetics

Cite this

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abstract = "A 120-kDa protein that blocks DNA termini has been purified from extracts of HeLa cells. This protein inhibits the action of a number of enzymes that catalyze reactions involving the 5' and 3' ends of DNA (DNA ligase, 3' and 5' exonucleases, and DNA polymerase α). The 120-kDa protein blocks the synthesis of long DNA chains that are normally formed during simian virus 40 DNA replication, causing the accumulation of small DNA fragments. The effects of this protein can be reversed by the addition of proliferating cell nuclear antigen and other protein fractions (activators).",
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AU - Ishimi, Y.

AU - Hurwitz, J.

PY - 1989

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AB - A 120-kDa protein that blocks DNA termini has been purified from extracts of HeLa cells. This protein inhibits the action of a number of enzymes that catalyze reactions involving the 5' and 3' ends of DNA (DNA ligase, 3' and 5' exonucleases, and DNA polymerase α). The 120-kDa protein blocks the synthesis of long DNA chains that are normally formed during simian virus 40 DNA replication, causing the accumulation of small DNA fragments. The effects of this protein can be reversed by the addition of proliferating cell nuclear antigen and other protein fractions (activators).

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