Studies on the regulation of hemopoiesis

P. Quesenberry, T. Alberico, Z. Song, G. Donowitz, M. Stewart, R. Gualtieri, H. Boswell, E. McGrath, E. Kleeman, G. Baber

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Normal hemopoietic cell differentiation and proliferation is critically dependent upon marrow stromal elements. Long-term liquid culture of marrow provides a model for the study of stromal function. We evaluated the effects of radiation and 5-fluorouracil (5-FU) on various aspects of long-term murine hemopoietic cell growth and stromal function. Exposure of C57BL/6J murine adherent cells from long-term marrow cultures to varying doses of irradiation (0-1000 rad) in vitro resulted in the elaboration of growth factors stimulating granulocyte, macrophage, megakaryocyte, mixed megakaryocyte-granulocyte macrophage, and blast colonies. This increased production of growth factors appears to be related to the ablation of normal granulocyte production in the culture system since addition of normal stroma to irradiated stroma blocks growth factor production. Two cell types appear to be mediating stromal factor production and support of liquid culture hemopoiesis: a macrophagelike cell and an alkaline-phosphatase-positive epithelioid cell. Exposure of these two cell types to pokeweed mitogen results in marked enhancement of growth factor production. Furthermore, a cell line isolated from normal murine stroma produced an activity capable of acting at an early hemopoietic stem cell level and of inducing secondary marrow cell lines. The establishment of cultures from 5-FU-treated animals revealed that chemotherapy-depleted marrow was capable of establishing adequate stromal function and that the residual surviving stem cells had a higher than normal proliferative rate. In addition, the function of granulocytes derived from this post-5-FU marrow was normal. Thus, it appears that both chemotherapy and radiation exposure of marrow results in an enhanced capacity of stromal elements to produce growth factors and support hemopoiesis and that post-5-FU marrow represents an enriched source of high proliferative potential bone marrow stem cells.

Original languageEnglish (US)
Pages (from-to)43-48
Number of pages6
JournalExperimental Hematology
Volume13
Issue numberSUPPL. 16
StatePublished - 1985
Externally publishedYes

Fingerprint

Bone Marrow
Intercellular Signaling Peptides and Proteins
Granulocytes
Fluorouracil
Megakaryocytes
Stem Cells
Macrophages
Drug Therapy
Cell Line
Pokeweed Mitogens
Epithelioid Cells
Radiation Effects
Stromal Cells
Bone Marrow Cells
Alkaline Phosphatase
Cell Differentiation
Cell Proliferation
Growth

ASJC Scopus subject areas

  • Cancer Research
  • Cell Biology
  • Genetics
  • Hematology
  • Oncology
  • Transplantation

Cite this

Quesenberry, P., Alberico, T., Song, Z., Donowitz, G., Stewart, M., Gualtieri, R., ... Baber, G. (1985). Studies on the regulation of hemopoiesis. Experimental Hematology, 13(SUPPL. 16), 43-48.

Studies on the regulation of hemopoiesis. / Quesenberry, P.; Alberico, T.; Song, Z.; Donowitz, G.; Stewart, M.; Gualtieri, R.; Boswell, H.; McGrath, E.; Kleeman, E.; Baber, G.

In: Experimental Hematology, Vol. 13, No. SUPPL. 16, 1985, p. 43-48.

Research output: Contribution to journalArticle

Quesenberry, P, Alberico, T, Song, Z, Donowitz, G, Stewart, M, Gualtieri, R, Boswell, H, McGrath, E, Kleeman, E & Baber, G 1985, 'Studies on the regulation of hemopoiesis', Experimental Hematology, vol. 13, no. SUPPL. 16, pp. 43-48.
Quesenberry P, Alberico T, Song Z, Donowitz G, Stewart M, Gualtieri R et al. Studies on the regulation of hemopoiesis. Experimental Hematology. 1985;13(SUPPL. 16):43-48.
Quesenberry, P. ; Alberico, T. ; Song, Z. ; Donowitz, G. ; Stewart, M. ; Gualtieri, R. ; Boswell, H. ; McGrath, E. ; Kleeman, E. ; Baber, G. / Studies on the regulation of hemopoiesis. In: Experimental Hematology. 1985 ; Vol. 13, No. SUPPL. 16. pp. 43-48.
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