Studies on ultrastructural dislocations in mitochondria. II. On the dislocation induced by lyophilization and the mechanism of uncoupling

W. Jolly, R. A. Harris, J. Asai, G. Lenaz, D. E. Green

Research output: Contribution to journalArticle

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Lyophilization has been shown to uncouple bovine heart mitochondria; however, well-coupled particles may be derived by sonication of the mitochondria uncoupled in this way. An ultrastructural dislocation has been demonstrated by electron microscopy in the cristael membrane of the lyophilized particles. This dislocation is believed to be the morphological basis of uncoupling in lyophilized mitochondria. A mechanism is proposed whereby coupled submitochondrial particles may be derived by sonic irradiation of uncoupled mitochondria.

Original languageEnglish (US)
Pages (from-to)191-211
Number of pages21
JournalArchives of Biochemistry and Biophysics
Issue numberC
StatePublished - 1969


ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology

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