Lyophilization has been shown to uncouple bovine heart mitochondria; however, well-coupled particles may be derived by sonication of the mitochondria uncoupled in this way. An ultrastructural dislocation has been demonstrated by electron microscopy in the cristael membrane of the lyophilized particles. This dislocation is believed to be the morphological basis of uncoupling in lyophilized mitochondria. A mechanism is proposed whereby coupled submitochondrial particles may be derived by sonic irradiation of uncoupled mitochondria.
ASJC Scopus subject areas
- Molecular Biology