Studies on ultrastructural dislocations in mitochondria. II. On the dislocation induced by lyophilization and the mechanism of uncoupling

W. Jolly, Robert Harris, J. Asai, G. Lenaz, D. E. Green

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Lyophilization has been shown to uncouple bovine heart mitochondria; however, well-coupled particles may be derived by sonication of the mitochondria uncoupled in this way. An ultrastructural dislocation has been demonstrated by electron microscopy in the cristael membrane of the lyophilized particles. This dislocation is believed to be the morphological basis of uncoupling in lyophilized mitochondria. A mechanism is proposed whereby coupled submitochondrial particles may be derived by sonic irradiation of uncoupled mitochondria.

Original languageEnglish (US)
Pages (from-to)191-211
Number of pages21
JournalArchives of Biochemistry and Biophysics
Volume130
Issue numberC
StatePublished - 1969
Externally publishedYes

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Mitochondria
Freeze Drying
Submitochondrial Particles
Heart Mitochondria
Sonication
Electron Microscopy
Electron microscopy
Membranes
Irradiation

ASJC Scopus subject areas

  • Molecular Biology
  • Biophysics
  • Biochemistry

Cite this

Studies on ultrastructural dislocations in mitochondria. II. On the dislocation induced by lyophilization and the mechanism of uncoupling. / Jolly, W.; Harris, Robert; Asai, J.; Lenaz, G.; Green, D. E.

In: Archives of Biochemistry and Biophysics, Vol. 130, No. C, 1969, p. 191-211.

Research output: Contribution to journalArticle

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