Studying osteocyte function using the cell lines MLO-Y4 and MLO-A5

Jennifer Rosser, Lynda F. Bonewald

Research output: Chapter in Book/Report/Conference proceedingChapter

36 Scopus citations

Abstract

We describe the culture and use of MLO-Y4 cells in studies of gene expression, response to fluid flow, and dendrite growth. We also describe how to use the MLO-A5 cells as a model of osteoblast to osteocyte -differentiation and how to study their mineralization. These studies serve as a beginning point to study osteocyte functions and molecular mechanisms responsible for these functions.

Original languageEnglish (US)
Title of host publicationBone Research Protocols
EditorsMiep Helfrich, Stuart Ralston
Pages67-81
Number of pages15
DOIs
StatePublished - Jan 2 2012
Externally publishedYes

Publication series

NameMethods in Molecular Biology
Volume816
ISSN (Print)1064-3745

Keywords

  • Dendrites
  • E11
  • Fluid flow
  • MLO-A5
  • MLO-Y4
  • Mineralization
  • Osteocyte

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Fingerprint Dive into the research topics of 'Studying osteocyte function using the cell lines MLO-Y4 and MLO-A5'. Together they form a unique fingerprint.

  • Cite this

    Rosser, J., & Bonewald, L. F. (2012). Studying osteocyte function using the cell lines MLO-Y4 and MLO-A5. In M. Helfrich, & S. Ralston (Eds.), Bone Research Protocols (pp. 67-81). (Methods in Molecular Biology; Vol. 816). https://doi.org/10.1007/978-1-61779-415-5_6