Studying osteocyte function using the cell lines MLO-Y4 and MLO-A5

Jennifer Rosser, Lynda Bonewald

Research output: Chapter in Book/Report/Conference proceedingChapter

32 Citations (Scopus)

Abstract

We describe the culture and use of MLO-Y4 cells in studies of gene expression, response to fluid flow, and dendrite growth. We also describe how to use the MLO-A5 cells as a model of osteoblast to osteocyte -differentiation and how to study their mineralization. These studies serve as a beginning point to study osteocyte functions and molecular mechanisms responsible for these functions.

Original languageEnglish (US)
Title of host publicationBone Research Protocols
Pages67-81
Number of pages15
Volume816
DOIs
StatePublished - 2012
Externally publishedYes

Publication series

NameMethods in Molecular Biology
Volume816
ISSN (Print)10643745

Fingerprint

Osteocytes
Cell Line
Dendrites
Osteoblasts
Gene Expression
Growth

Keywords

  • Dendrites
  • E11
  • Fluid flow
  • Mineralization
  • MLO-A5
  • MLO-Y4
  • Osteocyte

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Rosser, J., & Bonewald, L. (2012). Studying osteocyte function using the cell lines MLO-Y4 and MLO-A5. In Bone Research Protocols (Vol. 816, pp. 67-81). (Methods in Molecular Biology; Vol. 816). https://doi.org/10.1007/978-1-61779-415-5_6

Studying osteocyte function using the cell lines MLO-Y4 and MLO-A5. / Rosser, Jennifer; Bonewald, Lynda.

Bone Research Protocols. Vol. 816 2012. p. 67-81 (Methods in Molecular Biology; Vol. 816).

Research output: Chapter in Book/Report/Conference proceedingChapter

Rosser, J & Bonewald, L 2012, Studying osteocyte function using the cell lines MLO-Y4 and MLO-A5. in Bone Research Protocols. vol. 816, Methods in Molecular Biology, vol. 816, pp. 67-81. https://doi.org/10.1007/978-1-61779-415-5_6
Rosser J, Bonewald L. Studying osteocyte function using the cell lines MLO-Y4 and MLO-A5. In Bone Research Protocols. Vol. 816. 2012. p. 67-81. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-61779-415-5_6
Rosser, Jennifer ; Bonewald, Lynda. / Studying osteocyte function using the cell lines MLO-Y4 and MLO-A5. Bone Research Protocols. Vol. 816 2012. pp. 67-81 (Methods in Molecular Biology).
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